Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada, C1A 4P3;
IMA Fungus. 2012 Dec;3(2):189-201. doi: 10.5598/imafungus.2012.03.02.11. Epub 2012 Dec 11.
Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences. Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove. The isolate was obtained from a red alga (Polysiphonia sp.) collected from the tidal zone in Canada at low tide. Organic extracts enriched in extrolites, obtained from fermentation on a rice-based media, inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), S. warneri, and Proteus vulgaris. Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.
根据 ITS、nLSU rDNA 和 β-微管蛋白基因序列的形态学和系统发育分析，描述了肾形 Westerdykella Ebead & Overy sp. nov.。肾形 Westerdykella 的特征是产生闭囊壳状子囊果，其中包含小的球形至近球形的子囊，子囊内有 32 个、无隔膜、深色、明显肾形的子囊孢子，中央有凹痕。该分离株是从加拿大潮间带低潮时采集的一种红藻（多丝藻属）中获得的。从基于大米的培养基发酵中获得的富含外生代谢产物的有机提取物抑制了耐甲氧西林金黄色葡萄球菌（MRSA）、万古霉素耐药粪肠球菌（VRE）、华纳氏葡萄球菌和普通变形杆菌的生长。本文介绍了负责观察到的抗菌活性的化合物的鉴定、肾形 Westerdykella 的分类描述，以及基于宏观和微观形态特征的已知 Westerdykella 种的二项式关键。
