Institutes for Pharmaceutical Discovery, 23 Business Park Drive, Branford, Connecticut 06405, United States.
J Nat Prod. 2013 Feb 22;76(2):157-69. doi: 10.1021/np300568m. Epub 2013 Jan 28.
The monoglucuronides and sulfates of epicatechin, 3'-O-methylepicatechin, and 4'-O-methylepicatechin, respectively, were synthesized as authentic bioanalytical standards. Reversed-phase HPLC methods capable of baseline separation of the glucuronides and sulfates have been developed. Both the epicatechin glucuronides and sulfates were stable in the solid state when stored under ambient conditions and in aqueous solution when stored refrigerated. These results should prove invaluable to the research community as analytical standards as well as in future studies of the biological and pharmacological effects of epicatechin in humans.
分别合成了表儿茶素、3'-O-甲基表儿茶素和 4'-O-甲基表儿茶素的单葡糖苷酸和硫酸盐作为真实的生物分析标准。开发了能够基线分离葡糖苷酸和硫酸盐的反相高效液相色谱法。表儿茶素葡糖苷酸和硫酸盐在室温下储存于固态时以及在冷藏下储存于水溶液时均稳定。这些结果对于研究界来说应该是非常有价值的，因为它们不仅可用作分析标准，还可用作未来研究表儿茶素在人类中的生物学和药理学效应的工具。
