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Microb Cell Fact. 2010 Mar 9;9:15. doi: 10.1186/1475-2859-9-15.
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The glucose metabolite methylglyoxal inhibits expression of the glucose transporter genes by inactivating the cell surface glucose sensors Rgt2 and Snf3 in yeast.葡萄糖代谢物甲基乙二醛通过使酵母细胞表面的葡萄糖传感器Rgt2和Snf3失活来抑制葡萄糖转运蛋白基因的表达。
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Integration of transcriptional and posttranslational regulation in a glucose signal transduction pathway in Saccharomyces cerevisiae.酿酒酵母葡萄糖信号转导途径中转录调控与翻译后调控的整合
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Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux.酿酒酵母中新型HXT基因的鉴定揭示了单个己糖转运蛋白对糖酵解通量的影响。
Mol Microbiol. 1995 Apr;16(1):157-67. doi: 10.1111/j.1365-2958.1995.tb02400.x.
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Impact of assimilable nitrogen availability in glucose uptake kinetics in Saccharomyces cerevisiae during alcoholic fermentation.在酒精发酵过程中，可同化氮对酿酒酵母葡萄糖摄取动力学的影响。
Microb Cell Fact. 2012 Jul 30;11:99. doi: 10.1186/1475-2859-11-99.

引用本文的文献

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The glucose metabolite methylglyoxal inhibits expression of the glucose transporter genes by inactivating the cell surface glucose sensors Rgt2 and Snf3 in yeast.葡萄糖代谢物甲基乙二醛通过使酵母细胞表面的葡萄糖传感器Rgt2和Snf3失活来抑制葡萄糖转运蛋白基因的表达。
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2
Understanding the mechanism of glucose-induced relief of Rgt1-mediated repression in yeast.了解葡萄糖诱导的酵母 Rgt1 介导的抑制缓解的机制。
FEBS Open Bio. 2014 Jan 3;4:105-11. doi: 10.1016/j.fob.2013.12.004. eCollection 2014.
3
Endocytosis and vacuolar degradation of the yeast cell surface glucose sensors Rgt2 and Snf3.酵母细胞表面葡萄糖传感器 Rgt2 和 Snf3 的内吞作用和液泡降解。
J Biol Chem. 2014 Mar 7;289(10):7247-7256. doi: 10.1074/jbc.M113.539411. Epub 2014 Jan 22.

本文引用的文献

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Functional dissection of the glucose signaling pathways that regulate the yeast glucose transporter gene (HXT) repressor Rgt1.解析调控酵母葡萄糖转运蛋白基因（HXT）阻遏物 Rgt1 的葡萄糖信号通路的功能。
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DNA-binding properties of the yeast Rgt1 repressor.酵母Rgt1阻遏物的DNA结合特性
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Glucose as a hormone: receptor-mediated glucose sensing in the yeast Saccharomyces cerevisiae.作为一种激素的葡萄糖：酿酒酵母中受体介导的葡萄糖感知
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Immobilized DNA-binding assay, an approach for in vitro DNA-binding assay.固定化DNA结合测定，一种用于体外DNA结合测定的方法。
Anal Biochem. 2004 Nov 15;334(2):401-2. doi: 10.1016/j.ab.2004.06.045.
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On the origin of cancer cells.论癌细胞的起源。
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Specificity and regulation of DNA binding by the yeast glucose transporter gene repressor Rgt1.酵母葡萄糖转运蛋白基因阻遏物Rgt1对DNA结合的特异性及调控
Mol Cell Biol. 2003 Aug;23(15):5208-16. doi: 10.1128/MCB.23.15.5208-5216.2003.
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Function and regulation of yeast hexose transporters.酵母己糖转运蛋白的功能与调控
Microbiol Mol Biol Rev. 1999 Sep;63(3):554-69. doi: 10.1128/MMBR.63.3.554-569.1999.
8
Heterologous URA3MX cassettes for gene replacement in Saccharomyces cerevisiae.用于酿酒酵母基因替换的异源URA3MX盒式结构。
Yeast. 1999 Apr;15(6):507-11. doi: 10.1002/(SICI)1097-0061(199904)15:6<507::AID-YEA369>3.0.CO;2-P.
9
Rgt1p of Saccharomyces cerevisiae, a key regulator of glucose-induced genes, is both an activator and a repressor of transcription.酿酒酵母的Rgt1p是葡萄糖诱导基因的关键调节因子，它既是转录激活因子又是转录抑制因子。
Mol Cell Biol. 1996 Nov;16(11):6419-26. doi: 10.1128/MCB.16.11.6419.
10
A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae.一种在酿酒酵母中进行直接基因缺失的简单高效方法。
Nucleic Acids Res. 1993 Jul 11;21(14):3329-30. doi: 10.1093/nar/21.14.3329.

构建用于筛选抑制葡萄糖摄取和糖酵解药物的酵母菌株。

Construction of yeast strains useful for screening drugs that inhibit glucose uptake and glycolysis.

机构信息

Department of Biochemistry and Molecular Biology, George Washington University Medical Center, Washington, DC 20037, USA.

出版信息

Anal Biochem. 2013 May 1;436(1):53-4. doi: 10.1016/j.ab.2013.01.013. Epub 2013 Jan 26.

DOI:10.1016/j.ab.2013.01.013
PMID:23357239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3602138/
Abstract

The budding yeast Saccharomyces cerevisiae expresses different isoforms of glucose transporters (HXTs) in response to different levels of glucose. Here, we constructed reporter strains in which the nourseothricin (NAT) resistance gene is expressed under the control of the HXT1, 2, or 3 promoter. The resulting HXT-NAT reporter strains exhibited a strict growth dependence on glucose, and their growth could be easily controlled and optimized by adjusting glucose concentration, demonstrating the value of the reporter strains for studying the molecular basis of differential expression of HXT genes, as well as for screening drugs that inhibit glucose uptake and glycolysis.

摘要

budding 酵母酿酒酵母在不同葡萄糖水平下表达不同的葡萄糖转运蛋白（HXTs）同工型。在这里，我们构建了报告菌株，其中潮霉素抗性基因在 HXT1、2 或 3 启动子的控制下表达。所得 HXT-NAT 报告菌株表现出严格的葡萄糖生长依赖性，其生长可以通过调整葡萄糖浓度轻松控制和优化，表明报告菌株在研究 HXT 基因差异表达的分子基础以及筛选抑制葡萄糖摄取和糖酵解的药物方面具有价值。