构建用于筛选抑制葡萄糖摄取和糖酵解药物的酵母菌株。
Construction of yeast strains useful for screening drugs that inhibit glucose uptake and glycolysis.
机构信息
Department of Biochemistry and Molecular Biology, George Washington University Medical Center, Washington, DC 20037, USA.
出版信息
Anal Biochem. 2013 May 1;436(1):53-4. doi: 10.1016/j.ab.2013.01.013. Epub 2013 Jan 26.
Abstract
The budding yeast Saccharomyces cerevisiae expresses different isoforms of glucose transporters (HXTs) in response to different levels of glucose. Here, we constructed reporter strains in which the nourseothricin (NAT) resistance gene is expressed under the control of the HXT1, 2, or 3 promoter. The resulting HXT-NAT reporter strains exhibited a strict growth dependence on glucose, and their growth could be easily controlled and optimized by adjusting glucose concentration, demonstrating the value of the reporter strains for studying the molecular basis of differential expression of HXT genes, as well as for screening drugs that inhibit glucose uptake and glycolysis.
摘要
budding 酵母酿酒酵母在不同葡萄糖水平下表达不同的葡萄糖转运蛋白（HXTs）同工型。在这里，我们构建了报告菌株，其中潮霉素抗性基因在 HXT1、2 或 3 启动子的控制下表达。所得 HXT-NAT 报告菌株表现出严格的葡萄糖生长依赖性，其生长可以通过调整葡萄糖浓度轻松控制和优化，表明报告菌株在研究 HXT 基因差异表达的分子基础以及筛选抑制葡萄糖摄取和糖酵解的药物方面具有价值。
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