体外合成的丁型肝炎病毒RNA在培养细胞中启动基因组复制。
In vitro-synthesized hepatitis delta virus RNA initiates genome replication in cultured cells.
作者信息
Glenn J S, Taylor J M, White J M
机构信息
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0450.
出版信息
J Virol. 1990 Jun;64(6):3104-7. doi: 10.1128/JVI.64.6.3104-3107.1990.
Abstract
Monomers of the genomic strand of hepatitis delta virus RNA were transcribed in vitro and then delivered to NIH 3T3 fibroblasts by using a liposome fusion technique. After 7 days, genome replication was detected, but only in fibroblasts that stably expressed the delta antigen. Sequence analysis of the replicated products identified them as faithful copies of the hepatitis delta virus genome found in virions.
摘要
丁型肝炎病毒RNA基因组链的单体在体外转录，然后通过脂质体融合技术递送至NIH 3T3成纤维细胞。7天后，检测到基因组复制，但仅在稳定表达δ抗原的成纤维细胞中检测到。对复制产物的序列分析确定它们是在病毒粒子中发现的丁型肝炎病毒基因组的忠实拷贝。
相似文献
1
In vitro-synthesized hepatitis delta virus RNA initiates genome replication in cultured cells.体外合成的丁型肝炎病毒RNA在培养细胞中启动基因组复制。
J Virol. 1990 Jun;64(6):3104-7. doi: 10.1128/JVI.64.6.3104-3107.1990.
2
trans-dominant inhibition of human hepatitis delta virus genome replication.人丁型肝炎病毒基因组复制的反式显性抑制
J Virol. 1991 May;65(5):2357-61. doi: 10.1128/JVI.65.5.2357-2361.1991.
3
A specific base transition occurs on replicating hepatitis delta virus RNA.在复制丁型肝炎病毒核糖核酸时会发生特定的碱基转换。
J Virol. 1990 Mar;64(3):1021-7. doi: 10.1128/JVI.64.3.1021-1027.1990.
4
Hepatitis delta virus genome replication: a polyadenylated mRNA for delta antigen.丁型肝炎病毒基因组复制：一种编码丁型抗原的多聚腺苷酸化信使核糖核酸。
J Virol. 1990 Jul;64(7):3192-8. doi: 10.1128/JVI.64.7.3192-3198.1990.
5
Role of two forms of hepatitis delta virus antigen: evidence for a mechanism of self-limiting genome replication.两种形式的丁型肝炎病毒抗原的作用：自我限制基因组复制机制的证据。
J Virol. 1990 Oct;64(10):5066-9. doi: 10.1128/JVI.64.10.5066-5069.1990.
6
Initiation of replication of the human hepatitis delta virus genome from cloned DNA: role of delta antigen.从克隆DNA起始人类丁型肝炎病毒基因组的复制：丁型抗原的作用
J Virol. 1989 May;63(5):1945-50. doi: 10.1128/JVI.63.5.1945-1950.1989.
7
Replication of monomeric genomic delta RNA in cultured cells.
Prog Clin Biol Res. 1991;364:293-7.
8
Continuous expression and replication of the hepatitis delta virus genome in Hep G2 hepatoblastoma cells transfected with cloned viral DNA.在转染了克隆病毒DNA的Hep G2肝癌细胞中，丁型肝炎病毒基因组的持续表达和复制。
Proc Natl Acad Sci U S A. 1990 Jul;87(14):5253-7. doi: 10.1073/pnas.87.14.5253.
9
Baculovirus-directed high level expression of the hepatitis delta antigen in Spodoptera frugiperda cells.
J Gen Virol. 1991 Apr;72 ( Pt 4):833-42. doi: 10.1099/0022-1317-72-4-833.
10
Regulation of polyadenylation of hepatitis delta virus antigenomic RNA.丁型肝炎病毒反基因组RNA的聚腺苷酸化调控
J Virol. 1991 Dec;65(12):6438-46. doi: 10.1128/JVI.65.12.6438-6446.1991.
引用本文的文献
1
A general RNA-templated RNA extension activity of RNA polymerase.RNA聚合酶的一种普遍的以RNA为模板的RNA延伸活性。
RNA. 2025 Apr 16;31(5):663-678. doi: 10.1261/rna.080238.124.
2
N-Methyladenine Modification of Hepatitis Delta Virus Regulates Its Virion Assembly by Recruiting YTHDF1.N6-甲基腺嘌呤修饰调控乙型肝炎 delta 病毒衣壳组装的分子机制研究
J Virol. 2022 Oct 12;96(19):e0112422. doi: 10.1128/jvi.01124-22. Epub 2022 Sep 14.
3
Infection by Hepatitis Delta Virus.感染丁型肝炎病毒。
Viruses. 2020 Jun 16;12(6):648. doi: 10.3390/v12060648.
4
Hepatitis Delta Virus: Replication Strategy and Upcoming Therapeutic Options for a Neglected Human Pathogen.丁型肝炎病毒：一种被忽视的人类病原体的复制策略及未来治疗选择
Viruses. 2017 Jul 4;9(7):172. doi: 10.3390/v9070172.
5
A novel quantitative microarray antibody capture assay identifies an extremely high hepatitis delta virus prevalence among hepatitis B virus-infected mongolians.一种新型定量微阵列抗体捕获检测法确定了乙型肝炎病毒感染的蒙古人中丁型肝炎病毒的极高流行率。
Hepatology. 2017 Dec;66(6):1739-1749. doi: 10.1002/hep.28957. Epub 2017 Oct 30.
6
Hepatitis delta virus: insights into a peculiar pathogen and novel treatment options.丁型肝炎病毒：一种特殊病原体及其新型治疗方案的研究进展。
Nat Rev Gastroenterol Hepatol. 2016 Oct;13(10):580-9. doi: 10.1038/nrgastro.2016.126. Epub 2016 Aug 18.
7
Pathogen receptor discovery with a microfluidic human membrane protein array.利用微流控人膜蛋白阵列发现病原体受体
Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):4344-9. doi: 10.1073/pnas.1518698113. Epub 2016 Apr 4.
8
Construction and eukaryotic expression of recombinant large hepatitis delta antigen.重组大丁型肝炎抗原的构建及真核表达
Rep Biochem Mol Biol. 2013 Oct;2(1):28-34.
9
Hepatitis D Virus Replication.丁型肝炎病毒复制
Cold Spring Harb Perspect Med. 2015 Nov 2;5(11):a021568. doi: 10.1101/cshperspect.a021568.
10
Molecular and clinical aspects of hepatitis D virus infections.丁型肝炎病毒感染的分子与临床方面
World J Virol. 2012 Jun 12;1(3):71-8. doi: 10.5501/wjv.v1.i3.71.
本文引用的文献
1
Delta hepatitis agent: structural and antigenic properties of the delta-associated particle.丁型肝炎病毒：丁型相关颗粒的结构和抗原特性
Infect Immun. 1984 Mar;43(3):1000-5. doi: 10.1128/iai.43.3.1000-1005.1984.
2
The delta agent.δ因子
Hepatology. 1983 Sep-Oct;3(5):729-37. doi: 10.1002/hep.1840030518.
3
A replication cycle for viroids and other small infectious RNA's.类病毒及其他小感染性RNA的复制周期
Science. 1984 Feb 3;223(4635):450-5. doi: 10.1126/science.6197756.
4
Dissociation and reconstitution of the transcriptase and template activities of vesicular stomatitis B and T virions.水疱性口炎B和T病毒粒子转录酶及模板活性的解离与重组
J Virol. 1972 Aug;10(2):297-309. doi: 10.1128/JVI.10.2.297-309.1972.
5
Mechanisms of spontaneous and induced frameshift mutation in bacteriophage T4.噬菌体T4中自发和诱导移码突变的机制。
Genetics. 1985 Apr;109(4):633-59. doi: 10.1093/genetics/109.4.633.
6
Structure, sequence and expression of the hepatitis delta (delta) viral genome.丁型肝炎病毒基因组的结构、序列与表达
Nature. 1986;323(6088):508-14. doi: 10.1038/323508a0.
7
Replication of human hepatitis delta virus in primary cultures of woodchuck hepatocytes.人丁型肝炎病毒在土拨鼠肝细胞原代培养物中的复制。
J Virol. 1987 Sep;61(9):2891-5. doi: 10.1128/JVI.61.9.2891-2895.1987.
8
Human hepatitis delta antigen is a nuclear phosphoprotein with RNA-binding activity.人丁型肝炎抗原是一种具有RNA结合活性的核磷蛋白。
J Virol. 1988 Jul;62(7):2403-10. doi: 10.1128/JVI.62.7.2403-2410.1988.
9
Molecular cloning of hepatitis delta virus RNA from an infected woodchuck liver: sequence, structure, and applications.
J Virol. 1988 Jun;62(6):1855-61. doi: 10.1128/JVI.62.6.1855-1861.1988.
10
Characterization of self-cleaving RNA sequences on the genome and antigenome of human hepatitis delta virus.人丁型肝炎病毒基因组和反基因组上自我切割RNA序列的特征分析。
J Virol. 1988 Dec;62(12):4439-44. doi: 10.1128/JVI.62.12.4439-4444.1988.
Zotero 插件