Department of General Surgery, Nanfang Hospital, Southern Medical University, No. 1838 Guangzhou Avenue North, Guangzhou 510515, China.
Med Oncol. 2013 Mar;30(1):473. doi: 10.1007/s12032-013-0473-3. Epub 2013 Jan 29.
Colorectal cancer (CRC) patients with peritoneal carcinomatosis (PC) have a poor prognosis. Over the last decade, increasing evidence has suggested that the Collagen Triple Helix Repeat Containing 1 (CTHRC1) gene is involved in cancer progression and invasion. In this study, we investigated the expression of CTHRC1 in CRC and its potential as a prognostic factor for CRC patients with PC. Microarray analysis of four fresh paired samples showed that the expression of CTHRC1 in peritoneal metastases was higher than that in the corresponding primary tumor. These results were validated using semi-quantitative reverse transcription and polymerase chain reaction. Finally, immunohistochemical analysis showed that CTHRC1 was increased in the peritoneal metastasis group (n = 30) and the primary cancer with peritoneal metastasis group (n = 57) compared to the primary cancer without peritoneal metastasis group (n = 54), both P < 0.001. Cox proportional hazards model analysis showed that high CTHRC1 expression was associated with poor survival (HR = 2.754, P < 0.001, 95 % CI 1.731-4.383). Overall, the results of our study suggest that increased expression of CTHRC1 is associated with PC in CRC patients and could predict poor outcome in CRC patients.
结直肠癌（CRC）合并腹膜转移（PC）的患者预后较差。过去十年间，越来越多的证据表明 Collagen Triple Helix Repeat Containing 1（CTHRC1）基因参与了癌症的进展和侵袭。在本研究中，我们调查了 CTHRC1 在 CRC 中的表达情况及其作为 CRC 合并 PC 患者预后因素的潜在价值。对四个新鲜配对样本的微阵列分析表明，腹膜转移组织中 CTHRC1 的表达高于相应的原发性肿瘤。通过半定量逆转录和聚合酶链反应进行了验证。最后，免疫组化分析显示，与无腹膜转移的原发性肿瘤组（n=54）相比，腹膜转移组（n=30）和原发性肿瘤合并腹膜转移组（n=57）中 CTHRC1 表达增加，均 P<0.001。Cox 比例风险模型分析显示，高 CTHRC1 表达与不良预后相关（HR=2.754，P<0.001，95%CI 1.731-4.383）。总之，本研究结果表明，CTHRC1 的表达增加与 CRC 患者的 PC 相关，并可能预测 CRC 患者的不良结局。
