Remotti Helen
Department of Pathology and Cell Biology, Columbia University Medical Center, New York, NY, USA.
Methods Mol Biol. 2013;980:13-28. doi: 10.1007/978-1-62703-287-2_2.
Tissue microarrays (TMAs) enable high-throughput tissue analysis by selecting a large number of -paraffin-embedded donor tissue block cores and transferring these tissue cores into a positionally encoded array in the recipient TMA block. Once TMAs are constructed, a variety of analysis may be performed on the arrays including histochemical, immunohistochemical, or immunofluorescent staining, and in situ hybridization for DNA or RNA. TMAs offer a cost-effective method for performing parallel analysis of a large number of tissue samples. In this chapter we outline the method of TMA construction with an emphasis on providing useful information in the analysis of a variety of pancreatic neoplasms, including pancreatic adenocarcinomas and pre-invasive lesions. The technique of TMA construction in this chapter is restricted to the use of formalin-fixed paraffin-embedded tissue.
组织微阵列（TMAs）通过选择大量石蜡包埋的供体组织块芯，并将这些组织芯转移到受体TMA块中的位置编码阵列中，实现高通量组织分析。一旦构建好TMAs，就可以对阵列进行多种分析，包括组织化学、免疫组织化学或免疫荧光染色，以及DNA或RNA的原位杂交。TMAs为大量组织样本的平行分析提供了一种经济高效的方法。在本章中，我们概述了TMA构建方法，重点是在分析各种胰腺肿瘤（包括胰腺腺癌和癌前病变）时提供有用信息。本章中TMA构建技术仅限于使用福尔马林固定石蜡包埋组织。
