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水飞蓟甲醇提取物的自由基清除能力:果实中花色苷和酚酸的组成。

Radical scavenging capacity of methanolic Phillyrea latifolia L. extract: anthocyanin and phenolic acids composition of fruits.

机构信息

Department of Chemistry, Akdeniz University, Antalya 07058, Turkey.

出版信息

Molecules. 2013 Jan 30;18(2):1798-810. doi: 10.3390/molecules18021798.

Abstract

Radical scavenging capacity of a crude methanolic extract from the fruits of Phillyrea latifolia L., commonly known as green olive tree or mock privet, was investigated with reference to anthocyanin standards, as flavonoids, and phenolic acid standards, as phenylpropanoids. Characterization with high performance liquid chromatography-diode array detection (HPLC-DAD) indicated the presence of keracyanin, kuromanin, cyanidin, ferulic acid, caffeic acid and rosmarinic acid at amounts of 289.1, 90.4, 191.4, 225.2, 221.2 and 190.1 mg/100 g fresh weight (FW) of fruits, respectively. Chlorogenic and p-coumaric acids were found to exist in lower amounts. Trolox equivalent antioxidant capacity (TEAC) and IC(50) values of the plant extract were found to be 1.8 mM Trolox equivalents (TE)/g FW of fruits and 69.4 µg/mL, respectively, indicating the close radical scavenging activity of the extract to those of keracyanin and p-coumaric acid. The crude methanolic P. latifolia L. fruit extract was seen to be fairly potent in radical scavenging. Total phenolic content (TPC) of the plant extract was found to be 1652.9 mg gallic acid equivalent (GAE)/100 g FW of fruits.

摘要

从常绿树橄榄或假女贞果实中提取的粗甲醇提取物的自由基清除能力,参照类黄酮的花青素标准和苯丙素的酚酸标准进行了研究。高效液相色谱-二极管阵列检测(HPLC-DAD)的特征表明,果实中存在 keracyanin、kuromanin、花青素、阿魏酸、咖啡酸和迷迭香酸,其含量分别为 289.1、90.4、191.4、225.2、221.2 和 190.1mg/100g 鲜重(FW)。绿原酸和对香豆酸的含量较低。植物提取物的 Trolox 当量抗氧化能力(TEAC)和 IC50 值分别为 1.8mM Trolox 当量(TE)/g FW 的果实和 69.4μg/mL,表明提取物的自由基清除活性与 keracyanin 和对香豆酸的活性密切相关。粗甲醇法从常绿树橄榄或假女贞果实中提取的提取物具有相当强的自由基清除能力。植物提取物的总酚含量(TPC)为 1652.9mg 没食子酸当量(GAE)/100g FW 的果实。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f02b/6270162/998d596f3d7c/molecules-18-01798-g001.jpg

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