Creath Katherine, Goldstein Goldie
4D Technology Corporation, Tucson, AZ 85706, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2012;2012:3163-6. doi: 10.1109/EMBC.2012.6346636.
This paper describes recent advances in enhancing optical imaging performance and removal of background shape for a new, novel interference dynamic microscope system. The specially designed optical system enables instantaneous 4-dimensional video measurements of dynamic motions within and among live cells without the need for labels or contrast agents. This instrument utilizes a pixelated phase mask enabling simultaneous measurement of multiple interference patterns. It incorporates the polarization properties of light to capture phase image movies in real time at video rates enabling tracking of dynamic motions and volumetric changes. Optical thickness data are obtained from phase images after processing to remove the background surface shape to quantify changes in cell position and volume. Data from a number of different biological organisms will be presented. These data highlight examples of the optical image quality and image processing.
本文描述了一种新型干涉动态显微镜系统在增强光学成像性能和去除背景形状方面的最新进展。该特殊设计的光学系统能够对活细胞内部和之间的动态运动进行瞬时四维视频测量，而无需使用标记物或造影剂。该仪器利用像素化相位掩膜能够同时测量多个干涉图案。它结合了光的偏振特性，以视频速率实时捕获相位图像电影，从而能够跟踪动态运动和体积变化。在处理后从相位图像中获取光学厚度数据，以去除背景表面形状，从而量化细胞位置和体积的变化。将展示来自多种不同生物有机体的数据。这些数据突出了光学图像质量和图像处理的示例。
