Hassoun J, Monges G, Giraud P, Henry J F, Charpin C, Payan H, Toga M
Am J Pathol. 1984 Jan;114(1):56-63.
Immunohistochemical investigations were carried out on 16 pheochromocytomas for a study of their immunoreactivity to methionine-enkephalin, vasoactive intestinal peptide, somatostatin, corticotropin, beta-endorphin, and calcitonin on serial semithin araldite sections. All antiserums except anti-beta-endorphin, selectively stained a variable number of distinct tumor cells. Methionine-enkephalin-immunoreactive cells were the most frequent. The neuropeptide content of pheochromocytomas appears highly diverse and unpredictable. These findings are supportive of the concept of multisecretory APUD cells of neural crest origin and rule out any of these neuropeptides as reliable immunohistochemical markers for tumor chromaffin cells.
对16例嗜铬细胞瘤进行了免疫组织化学研究，以研究其在连续半薄环氧树脂切片上对甲硫氨酸脑啡肽、血管活性肠肽、生长抑素、促肾上腺皮质激素、β-内啡肽和降钙素的免疫反应性。除抗β-内啡肽外，所有抗血清均选择性地染色了数量不等的不同肿瘤细胞。甲硫氨酸脑啡肽免疫反应性细胞最为常见。嗜铬细胞瘤的神经肽含量似乎高度多样化且不可预测。这些发现支持了神经嵴起源的多分泌APUD细胞的概念，并排除了这些神经肽作为肿瘤嗜铬细胞可靠免疫组织化学标志物的可能性。
