Crypto-Giardia 抗原快速检测与传统改良 Ziehl-Neelsen 酸染色法诊断隐孢子虫病的比较。

Crypto-Giardia antigen rapid test versus conventional modified Ziehl-Neelsen acid fast staining method for diagnosis of cryptosporidiosis.

机构信息

Parasitology Department, Assuit University, Assuit, Egypt.

出版信息

Asian Pac J Trop Med. 2013 Mar;6(3):212-5. doi: 10.1016/S1995-7645(13)60025-5.

DOI:10.1016/S1995-7645(13)60025-5

PMID:23375035

Abstract

OBJECTIVE

To evaluate the validity of Crypto-Giardia antigen rapid test (CA-RT) in comparison with the conventional modified Ziehl-Neelsen acid fast (MZN-AF) staining method for the diagnosis of cryptosporidiosis.

METHODS

Fifteen preserved stool samples from previously confirmed infections were used as positive controls and 40 stool samples from healthy people were used as negative control. A total of 85 stool samples were collected from suspected patients with cryptosporidiosis over 6 months during the period from January till June, 2011. The study was conducted in the department of parasitology, central laboratory, Alnoor Specialist Hospital, Makkah, Saudi Arabia. All samples were subjected to CA-RT and conventional MZN-AF staining method. Validation parameters including sensitivity (SN), specificity (SP), accuracy index (AI), positive predictive value (PPV), and negative predictive value (NPV) were evaluated for both tests.

RESULTS

Out of 15 positive controls, CA-RT detected 13 (86.7%) while MZN-AF detected 11(73.3%) positive cases. However, CA-RT detected no positive case in 40 normal controls but MZN-AF detected 2(5%) as positive cases. Based on the results, the SN, SP, AI, PPV and NPV were high in CA-RT than MZN-AF staining method, ie., 86.7%vs. 73.3%, 100%vs. 95%, 96.4%vs. 89.1%, 100%vs. 84.6% and 95.2%vs. 90.5%, respectively. Out of a total of 85 suspected specimens, CA-RT detected 7(8.2%) but MZN-AF detected 6(7.1%) cases as positive.

CONCLUSIONS

CA-RT immunoassay is more valid and reliable than MZN-AF staining method.

摘要

目的

评估 Crypto-Giardia 抗原快速检测（CA-RT）与传统改良齐氏抗酸染色（MZN-AF）法在诊断隐孢子虫病方面的有效性。

方法

使用 15 份先前证实感染的保存粪便样本作为阳性对照，40 份健康人粪便样本作为阴性对照。2011 年 1 月至 6 月期间，在沙特阿拉伯麦加的 Alnoor 专科医院寄生虫学系和中央实验室共收集了 85 份疑似隐孢子虫病患者的粪便样本。所有样本均进行 CA-RT 和常规 MZN-AF 染色法检测。评估两种检测方法的验证参数，包括敏感性（SN）、特异性（SP）、准确性指数（AI）、阳性预测值（PPV）和阴性预测值（NPV）。

结果

在 15 份阳性对照中，CA-RT 检测出 13 份（86.7%）阳性病例，而 MZN-AF 检测出 11 份（73.3%）阳性病例。然而，CA-RT 在 40 份正常对照中未检测到阳性病例，但 MZN-AF 检测出 2 份（5%）为阳性病例。根据结果，CA-RT 的 SN、SP、AI、PPV 和 NPV 均高于 MZN-AF 染色法，分别为 86.7%vs.73.3%、100%vs.95%、96.4%vs.89.1%、100%vs.84.6%和 95.2%vs.90.5%。在总共 85 份疑似标本中，CA-RT 检测出 7 份（8.2%）阳性病例，而 MZN-AF 检测出 6 份（7.1%）阳性病例。