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Use of the UV ParaLens adapter as an alternative to conventional fluorescence microscopy for detection of Pneumocystis carinii in direct immunofluorescent monoclonal antibody-stained pulmonary specimens.使用紫外线平行透镜适配器替代传统荧光显微镜，用于在直接免疫荧光单克隆抗体染色的肺部标本中检测卡氏肺孢子虫。
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Quantitation of Giardia cysts and Cryptosporidium oocysts in fecal samples by direct immunofluorescence assay.通过直接免疫荧光测定法对粪便样本中的贾第虫囊肿和隐孢子虫卵囊进行定量分析。
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Three-step stool examination for cryptosporidiosis in 10 homosexual men with protracted watery diarrhea.对10名患有持续性水样腹泻的同性恋男性进行隐孢子虫病的三步粪便检查。
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直接免疫荧光法与传统染色法检测人体粪便标本中贾第虫和隐孢子虫属的前瞻性比较

Prospective comparison of direct immunofluorescence and conventional staining methods for detection of Giardia and Cryptosporidium spp. in human fecal specimens.

作者信息

Alles A J, Waldron M A, Sierra L S, Mattia A R

机构信息

Department of Pathology, Massachusetts General Hospital, Boston 02114, USA.

出版信息

J Clin Microbiol. 1995 Jun;33(6):1632-4. doi: 10.1128/jcm.33.6.1632-1634.1995.

DOI:10.1128/jcm.33.6.1632-1634.1995
PMID:7544365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC228230/
Abstract

In a prospective comparative study, 2,696 consecutive fresh stool specimens over the course of 1 year were examined for Giardia lamblia and Cryptosporidium parvum by using a direct immunofluorescent-monoclonal antibody stain (for unspun specimens) and conventional staining methods (chlorazol black E for Giardia cysts and modified Kinyoun acid-fast for Cryptosporidium oocysts). The direct immunofluorescent-monoclonal antibody method resulted in a significantly increased detection rate for both giardia (118 versus 79 specimens, 49.4%; P = 0.006) and cryptosporidia (39 versus 23 specimens, 69.6%; P = 0.055).

摘要

在一项前瞻性比较研究中，在1年的时间里，连续对2696份新鲜粪便标本进行了检测，采用直接免疫荧光单克隆抗体染色法（针对未离心标本）和传统染色方法（用氯唑黑E检测贾第虫包囊，用改良金胺酚抗酸染色法检测隐孢子虫卵囊）检测蓝氏贾第鞭毛虫和微小隐孢子虫。直接免疫荧光单克隆抗体法使贾第虫（118份标本对79份标本，49.4%；P = 0.006）和隐孢子虫（39份标本对23份标本，69.6%；P = 0.055）的检出率均显著提高。