Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy.
Am J Infect Control. 2013 Sep;41(9):836-40. doi: 10.1016/j.ajic.2012.09.021. Epub 2013 Feb 1.
Environmental contamination and transmission of methicillin-resistant Staphylococcus aureus (MRSA) have been reported in dental health care settings. National professional dental associations recommend controlling surface contamination using disposable barriers or disinfection. Because these procedures may be costly, impractical, and/or toxic, we compared their effect against traditional detergent-based cleaning for decontaminating a dental chair sprayed with MRSA.
Five MRSA strain suspensions were aerosolized to give a density of approximately 10 colony-forming units/cm(2) MRSA on the dental chair 5 minutes after dispersal. Three different decontamination protocols were applied: protocol 1: disposable barriers positioned before aerosol production and removed after 5 minutes; protocol 2: disinfection (wipe-rinse method) with 1:10 dilution of 5.25% to 6.15% sodium hypochlorite solution; protocol 3: cleaning (wipe-rinse method) with a sodium-lauryl-sulphate-based detergent. Contact plates containing Mannitol Salt Agar were used to assess the level of MRSA contamination.
All 3 protocols decreased MRSA surface load by >99%. Residual densities on the dental chair were 0.030 ± 0.010 (protocol 1), 0.029 ± 0.09 (protocol 2), and 0.030 ± 0.011 (protocol 3) colony-forming units/cm(2).
Cleaning (wipe-rinse method) using a sodium-lauryl-sulphate-based detergent demonstrated equivalence with disposable barrier placement or disinfection-based protocol for reducing MRSA contamination on dental chairs. This has practical and cost implications for controlling MRSA transmission in dental health care settings.
已报道在牙科保健环境中存在耐甲氧西林金黄色葡萄球菌（MRSA）的环境污染和传播。国家专业牙科协会建议使用一次性屏障或消毒来控制表面污染。由于这些程序可能昂贵、不切实际和/或有毒，我们比较了它们对传统基于清洁剂的清洁方法对喷洒 MRSA 的牙科椅进行去污的效果。
将 5 种 MRSA 菌株悬浮液雾化，使在分散后 5 分钟时牙科椅上的 MRSA 密度约为 10 个菌落形成单位/cm(2)。应用了 3 种不同的去污方案：方案 1：在气溶胶产生前放置一次性屏障，并在 5 分钟后移除；方案 2：用 5.25%至 6.15%次氯酸钠溶液稀释 1:10 的消毒（擦拭-冲洗法）；方案 3：用基于十二烷基硫酸钠的清洁剂进行清洁（擦拭-冲洗法）。使用含甘露醇盐琼脂的接触平板来评估 MRSA 污染水平。
所有 3 种方案均使 MRSA 表面负荷降低了 >99%。牙科椅上的残留密度分别为 0.030 ± 0.010（方案 1）、0.029 ± 0.09（方案 2）和 0.030 ± 0.011（方案 3）菌落形成单位/cm(2)。
使用基于十二烷基硫酸钠的清洁剂进行清洁（擦拭-冲洗法）与一次性屏障放置或基于消毒的方案等效，可减少牙科椅上 MRSA 的污染。这对控制牙科保健环境中 MRSA 的传播具有实际和成本意义。
