Ryvniak V V, Gudumak V S, Onia E S
Biull Eksp Biol Med. 1990 Feb;109(2):199-200.
The distribution of cathepsin D in liver with CCl4 induced cirrhosis and its involution in rats was investigated by ultrastructural cytochemistry. Besides intracellular, it was revealed the extracellular activity of cathepsin D. The reaction product was on collagen fibers near the hepatocytes and connective tissue cells as well as on the hepatocytes microvilli and on the outside part of cellular membrane of connective tissue cells (macrophage, fibroblast, Ito cells). Hence the source of extracellular cathepsin D in liver are the parenchymatous as well as nonparenchymal cell elements. The results testify that under the cirrhosis and its involution, the cathepsin D takes part in intracellular proteolysis and is secreted by hepatocytes and connective tissue cells in the intracellular space; it also takes part in extracellular catabolism of connective tissue.
通过超微结构细胞化学研究了组织蛋白酶D在四氯化碳诱导的大鼠肝硬化肝脏中的分布及其消退情况。除细胞内之外，还发现了组织蛋白酶D的细胞外活性。反应产物存在于肝细胞和结缔组织细胞附近的胶原纤维上，以及肝细胞微绒毛和结缔组织细胞（巨噬细胞、成纤维细胞、伊托细胞）细胞膜的外部。因此，肝脏中细胞外组织蛋白酶D的来源是实质细胞以及非实质细胞成分。结果证明，在肝硬化及其消退过程中，组织蛋白酶D参与细胞内蛋白水解，并由肝细胞和结缔组织细胞分泌到细胞内空间；它还参与结缔组织的细胞外分解代谢。
