Ryvniak V V
Biull Eksp Biol Med. 1986 Dec;102(12):760-3.
Electron microscopy and electron histochemistry (exposure to acid phosphatase) were used to study the mechanisms of extracellular degradation of collagen in the liver during involution of experimental cirrhosis. The following results were obtained: extracellular secretion of lysosomal enzymes from hepatocytes and connective tissue cells takes place in liver cirrhosis and its involution; partial hepatectomy during liver cirrhosis stimulates the activity of acid phosphatase in the liver cells; the lysosomal enzymes, excreted from hepatocytes and connective tissue cells by means of exocytosis take an active part in collagen extracellular degradation in vivo; at initial stages of cirrhosis involution extracellular degradation of collagen in the liver occurs at the expense of lysosomal enzymes from hepatocytes and connective tissue cells. Subsequently, as cirrhosis regresses, the principal role in the lysis of collagen gradually passes to lysosomal enzymes of hepatocytes.
采用电子显微镜和电子组织化学方法（酸性磷酸酶染色）研究实验性肝硬化消退过程中肝脏内胶原蛋白细胞外降解的机制。获得以下结果：在肝硬化及其消退过程中，肝细胞和结缔组织细胞会发生溶酶体酶的细胞外分泌；肝硬化期间进行部分肝切除术可刺激肝细胞中酸性磷酸酶的活性；通过胞吐作用从肝细胞和结缔组织细胞排出的溶酶体酶在体内胶原蛋白的细胞外降解中起积极作用；在肝硬化消退的初始阶段，肝脏中胶原蛋白的细胞外降解是由肝细胞和结缔组织细胞的溶酶体酶介导的。随后，随着肝硬化的消退，胶原蛋白溶解的主要作用逐渐转移至肝细胞的溶酶体酶。
