钙在莱茵衣藻鞭毛缩短和再生过程中的作用。
Participation of calcium in flagellar shortening and regeneration in Chlamydomonas reinhardii.
作者信息
Quader H, Cherniack J, Filner P
机构信息
Michigan State University-ERDA Plant Research Laboratory, East Lansing 48824.
出版信息
Exp Cell Res. 1978 May;113(2):295-301. doi: 10.1016/0014-4827(78)90369-5.
Cation chelators cause flagellar shortening in Chlamydomonas reinhardii. Most effective are EDTA and EGTA (1 mM) but pyrophosphate (10 mM) also is effective. Addition of 5 mM Ca2+ after shortening caused by 4 mM EGTA results in flagellar regeneration. Other divalent cations can replace Ca2+ with the following relative activities: Ca2+ greater than Sr2+ = Mn2+ much greater than Ba2+ = Mg2+. Although the specific ion requirement to reverse shortening is not clear, it is possible that all of the ions act by displacing one bound cation, presumably Ca2+. A specific requirement for Ca2+ in flagellar regeneration could be demonstrated, however, because as little as 50 microM EGTA in the presence of 500 microM Mg2+ delayed regeneration and prevented full regeneration. Ca2+ at 100 microM overcame this inhibition.
阳离子螯合剂会导致莱茵衣藻的鞭毛缩短。最有效的是乙二胺四乙酸（EDTA）和乙二醇双四乙酸（EGTA，1 mM），但焦磷酸（10 mM）也有效。在由4 mM EGTA引起鞭毛缩短后添加5 mM Ca²⁺会导致鞭毛再生。其他二价阳离子可以替代Ca²⁺，其相对活性如下：Ca²⁺＞Sr²⁺ = Mn²⁺ ＞＞ Ba²⁺ = Mg²⁺。虽然逆转鞭毛缩短所需的特定离子尚不清楚，但所有这些离子可能都是通过取代一个结合的阳离子（可能是Ca²⁺）来发挥作用的。然而，可以证明鞭毛再生对Ca²⁺有特定需求，因为在500 microM Mg²⁺存在的情况下，低至50 microM的EGTA会延迟再生并阻止完全再生。100 microM的Ca²⁺可克服这种抑制作用。
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