三维任意轨迹扫描光声显微镜

Three-dimensional arbitrary trajectory scanning photoacoustic microscopy.

作者信息

Yeh Chenghung, Soetikno Brian, Hu Song, Maslov Konstantin I, Wang Lihong V

机构信息

Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, One Brookings Dr., St. Louis, MO 63130, USA.

出版信息

J Biophotonics. 2015 Apr;8(4):303-8. doi: 10.1002/jbio.201400055. Epub 2014 Jul 30.

DOI:10.1002/jbio.201400055

PMID:25077689

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4312269/

Abstract

We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO2 ) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO2 dynamics in response to switching from systemic hypoxia to hyperoxia. 3-DAT-scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method.

摘要

我们通过三维任意轨迹（3-DAT）扫描增强了光声显微镜技术，该技术能够在大视野（FOV）范围内快速对选定血管成像，并且尽管血管深度存在变化，仍能保持高信噪比（SNR）。我们证明，在小鼠耳部活体中，血红蛋白氧饱和度（sO2）和血流能够以比传统二维光栅扫描快67倍的帧率同时进行测量。我们还观察到了从全身低氧切换到高氧时sO2的动态变化。3-DAT扫描光声显微镜。三维扫描平台及方法的示意图。