基于数字微镜器件的光谱编码光声显微镜
Spectrally encoded photoacoustic microscopy using a digital mirror device.
机构信息
Washington University in St. Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, 1 Brookings Drive, St. Louis, Missouri 63130, USA.
出版信息
J Biomed Opt. 2012 Jun;17(6):066020. doi: 10.1117/1.JBO.17.6.066020.
Abstract
We have developed spectrally encoded photoacoustic microscopy using a digital mirror device for multi-wavelength tomography, which enables fast spectral imaging of optical absorption. The optical illumination wavelengths are multiplexed at a laser pulse repetition rate of ≈ 2 kHz. Liquid samples, whole blood, and blood vessels in mouse ears were imaged. Compared with internal wavelength tuning of a narrow-band laser, external wavelength tuning based on a digital mirror device improves the data acquisition speed of spectral photoacoustic microscopy. Compared with external wavelength scanning of a wide-band laser with the same pulse energy, spectral encoding improves the signal-to-noise ratio.
摘要
我们开发了一种使用数字微镜器件的光谱编码光声显微镜，用于多波长层析成像，可实现光吸收的快速光谱成像。光学照明波长在 ≈ 2 kHz 的激光脉冲重复率下进行多路复用。对液体样本、全血和小鼠耳朵中的血管进行了成像。与窄带激光的内部波长调谐相比，基于数字微镜器件的外部波长调谐提高了光谱光声显微镜的采集速度。与具有相同脉冲能量的宽带激光的外部波长扫描相比，光谱编码提高了信噪比。
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