Zhu Wei Xiang, Zhao Kun, Chu Sha Sha, Liu Zhi Long
Department of Entomology, China Agricultural University, Beijing, China.
J Arthropod Borne Dis. 2012 Dec;6(2):90-7. Epub 2012 Dec 31.
The efficacy of essential oil of Chenopodium ambrosioides flowering aerial parts and its three main active ingredients was evaluated against Blattella germanica male adults.
Composition of essential oil was determined by GC-MS. Topical application bioassay was used to evaluate contact toxicity of essential oil and three main components. Fumigant toxicity of essential oil and its main components was measured using a sealed space method.
Twenty-two components were identified in the essential oil and the main components were (Z)-ascaridole (29.7%), isoascaridole (13.0%), ρ-cymene (12.7%) and piperitone (5.0%). The essential oil and (Z)-ascaridole, isoascaridole and ρ-cymene possessed fumigant toxicity against male German cockroaches with LC(50) values of 4.13, 0.55, 2.07 and 6.92 mg/L air, respectively. Topical application bioassay showed that all the three compounds were toxic to male German cockroaches and (Z)-ascaridole was the strongest with a LD(50) value of 22.02 μg/adult while the crude oil with a LD(50) value of 67.46 μg/adult.
The essential oil from Chinese C. ambrosioides and its three main active ingredients may be explored as natural potential insecticides in the control of cockroaches.
评价了土荆芥开花地上部分的精油及其三种主要活性成分对德国小蠊雄成虫的功效。
采用气相色谱-质谱联用仪测定精油成分。采用点滴法生物测定评估精油及其三种主要成分的接触毒性。采用密闭空间法测定精油及其主要成分的熏蒸毒性。
从精油中鉴定出22种成分，主要成分有（Z）-蛔蒿素（29.7%）、异蛔蒿素（13.0%）、对伞花烃（12.7%）和胡椒酮（5.0%）。精油以及（Z）-蛔蒿素、异蛔蒿素和对伞花烃对德国小蠊雄虫具有熏蒸毒性，其LC50值分别为4.13、0.55、2.07和6.92mg/L空气。点滴法生物测定表明，这三种化合物对德国小蠊雄虫均有毒性，（Z）-蛔蒿素毒性最强，LD50值为22.02μg/成虫，而原油的LD50值为67.46μg/成虫。
土荆芥精油及其三种主要活性成分有望开发成为控制蟑螂的天然潜在杀虫剂。
