通过在聚合物胶束纳米颗粒冠层中高密度堆积实现耐核酸酶 DNA
Nuclease-resistant DNA via high-density packing in polymeric micellar nanoparticle coronas.
机构信息
Department of Chemistry and Biochemistry, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.
出版信息
ACS Nano. 2013 Feb 26;7(2):1379-87. doi: 10.1021/nn305030g. Epub 2013 Feb 4.
Abstract
Herein, we describe a polymeric micellar nanoparticle capable of rendering nucleic acids resistant to nuclease digestion. This approach relies on utilizing DNA as the polar headgroup of a DNA-polymer amphiphile in order to assemble well-defined, discrete nanoparticles. Dense packing of DNA in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation. We demonstrate the preparation, purification, and characterization of the nanoparticles, then describe their resistance to treatment with endo- and exonucleases including snake-venom phosphodiesterase (SVP), a common, general DNA digestion enzyme.
摘要
本文描述了一种能够使核酸抵抗核酸酶消化的聚合物胶束纳米粒子。该方法依赖于利用 DNA 作为 DNA-聚合物两亲物的极性头基，以组装具有明确结构的离散纳米粒子。在胶束冠部中 DNA 的密集堆积允许互补寡核苷酸杂交，同时阻止酶降解。我们展示了纳米粒子的制备、纯化和表征，然后描述了它们对包括蛇毒磷酸二酯酶 (SVP) 在内的内切酶和外切酶的抗性，SVP 是一种常见的通用 DNA 消化酶。
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