中国仓鼠（黑线仓鼠）雄性和雌性肾脏中α-半乳糖苷酶水平的差异。

Difference in renal alpha-galactosidase levels in male and female Chinese hamsters (Cricetulus griseus).

Chang A Y

Diabetes and Atherosclerosis Research, Upjohn Co., Kalamazoo, MI 49001.

Comp Biochem Physiol B. 1978;61(1):133-7. doi: 10.1016/0305-0491(78)90230-4.

Abstract

The activity of alpha-galactosidase was found to be significantly higher in the kidney of female than that of male Chinese hamsters in a highly inbred colony but its activity in liver, heart and spleen remained similar between female and male animals. 2. Partially purified renal alpha-galactosidase by sequential column chromatography on Sepharose 6B and DEAE-Sepharose CL-6B showed identical elution profiles, pH optima (4.5), KmS (4.4 mM) and heat-inactivation curves between enzymes of male and female animals. 3. Thus, the observed higher activity of renal alpha-galactosidase in the females was due to elevated enzyme concentration, not a result of enzyme polymorphism.

摘要

在一个高度近亲繁殖的中国仓鼠群体中，发现雌性仓鼠肾脏中的α-半乳糖苷酶活性显著高于雄性，但雌性和雄性动物肝脏、心脏和脾脏中的该酶活性保持相似。2. 通过在琼脂糖6B和DEAE-琼脂糖CL-6B上进行连续柱层析对肾脏α-半乳糖苷酶进行部分纯化后，发现雄性和雌性动物的酶具有相同的洗脱曲线、最适pH（4.5）、米氏常数（4.4 mM）和热失活曲线。3. 因此，观察到的雌性肾脏α-半乳糖苷酶活性较高是由于酶浓度升高，而非酶多态性的结果。