酮症中国仓鼠肾中β-半乳糖苷酶的减少
Reduction of beta-galactosidase in the ketotic Chinese hamster kidney.
作者信息
Chang A Y
出版信息
Horm Metab Res. 1978 Mar;10(2):139-41. doi: 10.1055/s-0028-1093460.
Kidneys from normal, diabetic-nonketotic and ketotic Chinese hamsters were homogenized, fractionated and assayed for beta-glucosidase, and beta-galactosidase activities. The kidneys of the ketotic animals were enlarged but the protein content in each subcellular fraction was similar in all three groups of animals. beta-Glucosidase was found chiefly in the soluble fraction and no difference was observed in these animals. beta-Galactosidase was distributed in both cytoplasmic and particulate fractions; difference in the specific activity of beta-galactosidase between control and ketotic animals was found in nuclear, lysosomal-mitochondrial, microsomal and soluble fractions.
将正常、非糖尿病酮症及糖尿病酮症中国仓鼠的肾脏进行匀浆、分级分离，并测定β-葡萄糖苷酶和β-半乳糖苷酶活性。糖尿病酮症动物的肾脏体积增大，但三组动物每个亚细胞组分中的蛋白质含量相似。β-葡萄糖苷酶主要存在于可溶性组分中，在这些动物中未观察到差异。β-半乳糖苷酶分布于细胞质和颗粒组分中；在细胞核、溶酶体-线粒体、微粒体和可溶性组分中发现，对照动物与糖尿病酮症动物之间β-半乳糖苷酶的比活性存在差异。
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