Cancer Research Institute, Xiangya Medical School, Central South University, Changsha, China ; Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Changsha, China ; Key Laboratory of Carcinogenesis, Ministry of Health, Changsha, China ; Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, China.
PLoS One. 2013;8(1):e53989. doi: 10.1371/journal.pone.0053989. Epub 2013 Jan 29.
STC1 is a glycoprotein hormone involved in calcium/phosphate (Pi) homeostasis. There is mounting evidence that STC1 is tightly associated with the development of cancer. But the function of STC1 in cancer is not fully understood. Here, we found that STC1 is down-regulated in Clinical tissues of cervical cancer compared to the adjacent normal cervical tissues (15 cases). Subsequently, the expression of STC1 was knocked down by RNA interference in cervical cancer CaSki cells and the low expression promoted cell growth, migration and invasion. We also found that STC1 overexpression inhibited cell proliferation and invasion of cervical cancer cells. Moreover, STC1 overexpression sensitized CaSki cells to drugs. Further, we showed that NF-κB p65 protein directly bound to STC1 promoter and activated the expression of STC1 in cervical cancer cells. Thus, these results provided evidence that STC1 inhibited cell proliferation and invasion through NF-κB p65 activation in cervical cancer.
STC1 是一种糖蛋白激素，参与钙/磷（Pi）稳态。越来越多的证据表明，STC1 与癌症的发展密切相关。但是，STC1 在癌症中的功能尚未完全阐明。在这里，我们发现与相邻的正常宫颈组织相比，宫颈癌临床组织中 STC1 的表达下调（15 例）。随后，通过 RNA 干扰在宫颈癌 CaSki 细胞中敲低 STC1 的表达，低表达促进细胞生长、迁移和侵袭。我们还发现 STC1 过表达抑制了宫颈癌细胞的增殖和侵袭。此外，STC1 过表达使 CaSki 细胞对药物敏感。此外，我们表明 NF-κB p65 蛋白直接与 STC1 启动子结合并激活宫颈癌细胞中 STC1 的表达。因此，这些结果表明 STC1 通过 NF-κB p65 激活抑制宫颈癌中的细胞增殖和侵袭。
