Schaeffer M C, Cochary E F, Sadowski J A
Western Human Nutrition Research Center, United States Department of Agriculture, Agricultural Research Service, San Francisco, California 94129.
J Am Coll Nutr. 1990 Apr;9(2):120-7. doi: 10.1080/07315724.1990.10720360.
Motor abnormalities have been observed in every species made vitamin B6 deficient, and have been detected and quantified early in vitamin B6 deficiency in young adult female Long-Evans rats with hind leg gait analysis. Our objective was to determine if hind leg gait analysis could be used to detect vitamin B6 deficiency in weanling (3 weeks) and aged (23 months) Fischer 344 male rats. Rats (n = 10 per group) were fed: the control diet ad libitum (AL-CON); the control diet devoid of added pyridoxine hydrochloride (DEF); or the control diet pair-fed to DEF (PF-CON). At 10 weeks, plasma pyridoxal phosphate concentration confirmed deficiency in both age groups. Gait abnormalities were detected in the absence of gross motor disturbances in both aged and weanling DEF rats at 2-3 weeks. Width of step was significantly reduced (16%, p less than 0.003) in DEF aged rats compared to AL- and PF-CON. This pattern of response was similar to that reported previously in young adult rats. In weanling rats, pair feeding alone reduced mean width of step (+/- SEM) by 25% compared to ad libitum feeding (2.7 +/- 0.1 vs 3.6 +/- 0.1 cm for PF- vs AL-CON, respectively, p less than 0.05). In DEF weanling rats, width (3.0 +/- 0.1 cm) was increased compared to PF-CON (11%, p less than 0.05) but decreased compared to AL-CON (16%, p less than 0.05). Width of step was significantly altered early in B6 deficiency in rats of different ages and strains and in both sexes.(ABSTRACT TRUNCATED AT 250 WORDS)
在每一种维生素B6缺乏的物种中都观察到了运动异常，并且通过对成年雌性Long-Evans大鼠后腿步态分析，在维生素B6缺乏早期就检测到并进行了量化。我们的目的是确定后腿步态分析是否可用于检测断奶（3周龄）和老龄（23月龄）Fischer 344雄性大鼠的维生素B6缺乏。大鼠（每组n = 10）被喂食：自由采食对照饮食（AL-CON）；不含添加盐酸吡哆醇的对照饮食（DEF）；或与DEF配对喂食的对照饮食（PF-CON）。在10周时，血浆磷酸吡哆醛浓度证实两个年龄组均存在缺乏。在2 - 3周时，在老龄和断奶的DEF大鼠中，在没有明显运动障碍的情况下检测到步态异常。与AL-CON和PF-CON相比，DEF老龄大鼠的步幅宽度显著减小（16%，p < 0.003）。这种反应模式与之前在成年大鼠中报道的相似。在断奶大鼠中，与自由采食相比，单独配对喂食使平均步幅宽度（±SEM）降低了25%（PF-CON与AL-CON分别为2.7±0.1 vs 3.6±0.1 cm，p < 0.05）。在DEF断奶大鼠中，步幅宽度（3.0±0.1 cm）与PF-CON相比增加了（11%，p < 0.05），但与AL-CON相比降低了（16%，p < 0.05）。在不同年龄、品系和性别的大鼠中，维生素B6缺乏早期步幅宽度就有显著改变。（摘要截短于250字）
