Halwachs-Baumann G, Katzensteiner S, Schnedl W, Pürstner P, Pieber T, Wilders-Truschnig M
Department of Laboratory Medicine, Karl Franzens University, Graz, Austria.
Clin Chem. 1997 Mar;43(3):511-7.
We evaluated three newly introduced systems for automated determinations of hemoglobin (Hb) A1c, which allow the processing of large amounts of samples in a routine clinical laboratory. We compared these methods--the Variant HPLC, the Hi-Auto A1c analyzer system, and the Roche immunoassay--with the Diamat HPLC system. All showed good precision and good concordance with the Diamat HPLC. The reference range for Hb A1c has to be determined by the laboratory for each assay system. Interference study showed no statistically significant influence of anemia, polycythemia, rheumatoid factor, or chronic hemodialysis, although individual Hb A1c values can be influenced by polycythemia (when measured with the Hi-Auto A1c analyzer) and by chronic hemodialysis (when measured with the Variant HPLC). HPLC was not suitable for measuring Hb A1c in the examined cases of hemoglobin variants; assaying fructosamine seems to be better for monitoring these patients.
我们评估了三种新引进的用于自动测定糖化血红蛋白（Hb）A1c的系统，这些系统可在常规临床实验室处理大量样本。我们将这些方法——Variant高效液相色谱法（HPLC）、Hi-Auto A1c分析仪系统和罗氏免疫测定法——与Diamat HPLC系统进行了比较。所有方法均显示出良好的精密度，且与Diamat HPLC法具有良好的一致性。每个检测系统的Hb A1c参考范围必须由实验室确定。干扰研究表明，贫血、红细胞增多症、类风湿因子或慢性血液透析无统计学上的显著影响，尽管个别Hb A1c值可能受红细胞增多症（用Hi-Auto A1c分析仪测量时）和慢性血液透析（用Variant HPLC测量时）影响。在所检测的血红蛋白变异病例中，HPLC不适用于测量Hb A1c；检测果糖胺似乎更适合监测这些患者。
