Zhang Chunquan, Whitham Steven A, Hill John H
Department of Plant Pathology, Iowa State University, Ames, IA, USA.
Methods Mol Biol. 2013;975:149-56. doi: 10.1007/978-1-62703-278-0_11.
Plant viral vectors are useful for transient gene expression as well as for downregulation of gene expression via virus-induced gene silencing (VIGS). When used in reverse genetics approaches, VIGS offers a convenient way of transforming genomic information into knowledge of gene function. Efforts to develop and improve plant viral vectors have expanded their applications and have led to substantial advances needed to facilitate gene function studies in major row crops. Here, we describe a DNA-based Bean pod mottle virus (BPMV) vector system for both gene expression and VIGS in soybean and common bean.
植物病毒载体可用于瞬时基因表达，也可通过病毒诱导的基因沉默（VIGS）来下调基因表达。当用于反向遗传学方法时，VIGS为将基因组信息转化为基因功能知识提供了一种便捷的方式。开发和改进植物病毒载体的努力扩大了它们的应用范围，并带来了促进主要大田作物基因功能研究所需的重大进展。在此，我们描述了一种基于DNA的菜豆荚斑驳病毒（BPMV）载体系统，用于大豆和普通菜豆中的基因表达和VIGS。
