Hasegawa I, Takakuwa K, Adachi S, Kanazawa K
Department of Obstetrics and Gynecology, Niigata University School of Medicine, Japan.
J Reprod Immunol. 1990 Apr;17(2):127-39. doi: 10.1016/0165-0378(90)90031-z.
To elucidate immunological mechanisms involved in the genesis of intra-uterine fetal growth retardation (IUGR), an in vitro cytotoxicity assay against normal trophoblast and lymphocytes was performed. The data demonstrated the existence of cytotoxic antibody directed against trophoblast exclusively in the IgG fraction of the sera of 9 out of 15 mothers with IUGR, but in none of the sera from normal pregnant women. This antibody showed differential reactivity patterns that may be indicative women. This antibody showed differential reactivity patterns that may be trophoblast in common. Out of 9 IUGR mothers with this cytotoxic antibody, in 6 cases (66.7%) chronic villitis was evident upon microscopic examination of the placenta, the frequency being significantly higher than that in IUGR mothers without cytotoxicity or in control mothers (P less than 0.02). It is suggested that in situ inflammatory change triggered by this antibody might lead to IUGR through chronic villitis. This antibody showed cross-reactivity with anti-negatively charged phospholipid antibody, as confirmed by an absorption experiment, indicating that the trophoblast antigenic stimuli in pregnancy can induce the production of various autoantibodies including anti-phospholipid antibodies. These results are of interest in relation to the pathogenesis of autoimmune diseases.
为阐明宫内胎儿生长受限（IUGR）发生过程中的免疫机制，我们针对正常滋养层细胞和淋巴细胞进行了体外细胞毒性试验。数据显示，15例IUGR母亲中有9例血清的IgG部分存在仅针对滋养层细胞的细胞毒性抗体，而正常孕妇的血清中均未检测到。该抗体表现出不同的反应模式，这可能是共同滋养层细胞的特征。在9例具有这种细胞毒性抗体的IUGR母亲中，6例（66.7%）经胎盘显微镜检查显示有慢性绒毛炎，其发生率显著高于无细胞毒性的IUGR母亲或对照母亲（P<0.02）。提示该抗体引发的原位炎症变化可能通过慢性绒毛炎导致IUGR。吸收实验证实，该抗体与抗负电荷磷脂抗体存在交叉反应，表明妊娠时滋养层抗原刺激可诱导包括抗磷脂抗体在内的多种自身抗体产生。这些结果对于自身免疫性疾病发病机制的研究具有重要意义。
