Duguid J R, Dinauer M C
Geriatric Research, Education and Clinical Center, Edith N. Rogers Memorial Veterans Hospital, Bedford, MA 01730.
Nucleic Acids Res. 1990 May 11;18(9):2789-92. doi: 10.1093/nar/18.9.2789.
We have developed a system where double-stranded cDNA can be amplified using a synthetic oligonucleotide primer and the polymerase chain reaction, generating cDNA libraries in vitro. Using a library subtraction strategy (1), scrapie and control brain in vitro cDNA libraries were used to identify sequences whose expression is modulated in scrapie infection. One of these sequences represents beta-2 microglobulin, while the other two have not been previously described. The use of in vitro libraries offers increased speed and efficiency of construction, and their subtraction is more efficient and powerful, compared with the previous system (1).
我们开发了一种系统，其中双链cDNA可以使用合成寡核苷酸引物和聚合酶链反应进行扩增，从而在体外生成cDNA文库。采用文库消减策略（1），使用羊瘙痒病和对照脑的体外cDNA文库来鉴定其表达在羊瘙痒病感染中受到调节的序列。其中一个序列代表β-2微球蛋白，而另外两个序列以前未被描述过。与先前的系统（1）相比，体外文库的使用提高了构建的速度和效率，并且其消减更有效、更强大。
