Mestecky J, Winchester R J, Hoffman T, Kunkel H G
Rockefeller University, New York 10021.
J Exp Med. 1977 Mar 1;145(3):760-5. doi: 10.1084/jem.145.3.760.
The synthesis of intracellular J chains was found to be closely associated with that of intracellular immunoglobulin, regardless of its class, during the process of B-cell differentiation. This parallelism between the synthesis of J chain and immunoglobulin was particularly evident in their coincident appearance in serial observations of pokeweed mitogen (PWM)-stimulated lymphocytes. The intensity of J-chain staining by fluorescent reagents in the stimulated cells synthesizing IgG was similar to that found in cells synthesizing IgA or IgM. Evidence was obtained that the presence of J chain in the IgG-producing cells did not reflect antecedent synthesis of IgA or IgM. T cells stimulated by phytohemagglutinin and PWM failed to show J-chain synthesis. Observations on lymphoid cell lines showed a similar parallelism between intracellular Ig and J-chain synthesis; no relation to surface Ig was found.
在B细胞分化过程中,发现细胞内J链的合成与细胞内免疫球蛋白的合成密切相关,无论免疫球蛋白的类别如何。J链和免疫球蛋白合成之间的这种平行性在对商陆有丝分裂原(PWM)刺激的淋巴细胞的系列观察中,它们同时出现时尤为明显。在合成IgG的受刺激细胞中,荧光试剂对J链的染色强度与合成IgA或IgM的细胞中的染色强度相似。有证据表明,产生IgG的细胞中J链的存在并不反映IgA或IgM先前的合成。受植物血凝素和PWM刺激的T细胞未显示J链合成。对淋巴细胞系的观察表明,细胞内Ig和J链合成之间存在类似的平行性;未发现与表面Ig有关。