Stein U, Buschmann C, Blaich R, Lichtenthaler H K
Bundesforschungsanstalt für Rebenzüchtung, Siebeldingen, Federal Republic of Germany.
Radiat Environ Biophys. 1990;29(2):119-28. doi: 10.1007/BF01210557.
Induction kinetics of luminescence (= delayed chlorophyll fluorescence or delayed light emission) were measured with sun and shade leaves of a tall beech tree (Fagus sylvatica 'pendula', weeping beech). The kinetics detected in the ms-range are contrasted for the upper and the lower leaf side. The influence of the following parameters is demonstrated: time of dark-adaptation prior to the measurement, intensity of the excitation light and photoinhibitory treatment. The effects are discussed with respect to chlorophyll concentration, absorption of the excitation light, reabsorption of the luminescence and photosynthetic activity of the leaf tissue. It is shown that the luminescence signal and its kinetic are determined mainly by the properties of the mesophyll parenchyma facing the detector. Thus the more densely packed palisade parenchyma at the upper leaf side exhibits a lower luminescence and a slower kinetic than the spongy parenchyma at the lower leaf side, which is characterized by many aerial interspaces. Our study shows that luminescence kinetics can be applied to interpret the physiological state of a specific leaf tissue. They may serve as an indicator of disorders in the photosynthetic function.
利用一棵高大的山毛榉树（垂枝山毛榉，学名：Fagus sylvatica 'pendula'）的向阳叶和遮荫叶测量了发光（即延迟叶绿素荧光或延迟光发射）的诱导动力学。在毫秒范围内检测到的动力学情况针对叶片的上表面和下表面进行了对比。展示了以下参数的影响：测量前的暗适应时间、激发光强度和光抑制处理。结合叶绿素浓度、激发光吸收、发光再吸收以及叶片组织的光合活性对这些影响进行了讨论。结果表明，发光信号及其动力学主要由面向探测器的叶肉薄壁组织的特性决定。因此，叶片上表面排列更紧密的栅栏薄壁组织比下表面具有许多气腔间隙的海绵薄壁组织表现出更低的发光和更慢的动力学。我们的研究表明，发光动力学可用于解释特定叶片组织的生理状态。它们可作为光合功能紊乱的指标。
