Reproductive Biology and Toxicology Laboratory, School of Studies in Zoology, Jiwaji University, Gwalior, India.
J Environ Pathol Toxicol Oncol. 2012;31(4):325-34. doi: 10.1615/jenvironpatholtoxicoloncol.v31.i4.30.
In the present study, we evaluated the protective activity of an aqueous extract of Artemisia absinthium against CCl4-induced hepatic damage in rats. The protective activity of this extract at three doses (2.5, 5, and 10 ml/ kg, once orally) against CCl4-induced oxidative damage (1.5 ml/kg, once intraperitoneally) in rats was analyzed. Various blood and tissue biochemical studies were performed, and the administration of the toxicant significantly altered blood biochemical variables. Hepatic lipid peroxidation (LPO) was significantly elevated, whereas glutathione (GSH) level was considerable depleted after intoxication. Remarkable decreases in the activities of adenosine triphosphatase (ATPase) and glucose-6-phosphatase (G-6-Pase) after intoxication were observed. Treatment with all three doses reversed altered tissue biochemical values, but the greatest protection was observed at the lowest dose (2.5 ml/kg). The results of this study show that A. absinthium induces strong hepatoprotective activity. It decreased the hexobarbitone-induced sleep time and improved cholerectic activity (bile flow and bile solids) and excretory capacity, and it also stimulated bile secretion. The potent antioxidant activity of A. absinthium was indicated by scavenging effects on 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and hydrogen peroxide (H2O2). Thus, be considered for use in reducing hepatic damage and may serve as an alternative medicine in hepatic etiologies.
在本研究中，我们评估了苦艾水提取物对 CCl4 诱导的大鼠肝损伤的保护活性。分析了该提取物在三个剂量（2.5、5 和 10ml/kg，一次口服）对 CCl4 诱导的氧化损伤（1.5ml/kg，一次腹腔内注射）的保护活性。进行了各种血液和组织生化研究，发现给予有毒物质后，血液生化变量发生了显著改变。肝脂质过氧化（LPO）显著升高，而谷胱甘肽（GSH）水平在中毒后明显耗尽。中毒后，三磷酸腺苷酶（ATPase）和葡萄糖-6-磷酸酶（G-6-Pase）的活性显著下降。所有三种剂量的治疗均逆转了改变的组织生化值，但在最低剂量（2.5ml/kg）下观察到最大的保护作用。本研究结果表明，苦艾诱导了强烈的肝保护活性。它降低了戊巴比妥诱导的睡眠时间，改善了利胆活性（胆汁流量和胆汁固体）和排泄能力，还刺激了胆汁分泌。苦艾的强大抗氧化活性通过对 1,1-二苯基-2-苦基肼（DPPH）和过氧化氢（H2O2）的清除作用得到证实。因此，可考虑用于减少肝损伤，并可作为肝脏病因的替代药物。
