Vierhapper H
Division of Clinical Endocrinology and Diabetes Mellitus, I. Medizinische Universitätsklinik, Wien, Austria.
Steroids. 1990 Apr;55(4):177-80. doi: 10.1016/0039-128x(90)90107-m.
The determination of urinary 5 alpha-androstane-3 alpha,17 beta-diol (3a-Diol) by gas chromatography/mass spectometry during and after the infusion of stable-labeled testosterone (T) represents an alternative to the use of radioactive label for turnover studies in vivo. Using this methodology to assess the urinary excretion rates of T and 3a-Diol in healthy men (n = 6) and women (n = 5) during and after the intravenous infusion (t = 4 hours) of 20 mg (men) or 5 mg (women) [13C]testosterone, the cumulative renal excretion of 13C-labeled T was found to be 15.6 +/- 9.6 micrograms/24 hours (men) and 1.1 +/- 1.6 micrograms/24 hours (women), equivalent to 0.08% +/- 0.05% and 0.02% +/- 0.03% of the infused amount of 13C-T, respectively. The cumulative excretion of 13C-3a-Diol was 67.7 +/- 19.9 micrograms/24 hours (men) and 10.0 +/- 6.0 micrograms/24 hours (women), equivalent to 0.3 +/- 0.1% and 0.2 +/- 0.1% of the infused dose of 13C-labeled testosterone, respectively.
在输注稳定标记的睾酮（T）期间及之后，通过气相色谱/质谱法测定尿中5α-雄烷-3α,17β-二醇（3α-二醇），为体内周转率研究中使用放射性标记提供了一种替代方法。使用该方法评估20mg（男性）或5mg（女性）[13C]睾酮静脉输注（t = 4小时）期间及之后健康男性（n = 6）和女性（n = 5）尿中T和3α-二醇的排泄率，发现13C标记的T的累积肾排泄量为15.6±9.6微克/24小时（男性）和1.1±1.6微克/24小时（女性），分别相当于输注的13C-T量的0.08%±0.05%和0.02%±0.03%。13C-3α-二醇的累积排泄量为67.7±19.9微克/24小时（男性）和10.0±6.0微克/24小时（女性），分别相当于输注的13C标记睾酮剂量的0.3±0.1%和0.2±0.1%。
