牛对奥斯特他线虫幼虫抗原的免疫识别。

Bovine immune recognition of Ostertagia ostertagi larval antigens.

作者信息

Mansour M M, Dixon J B, Clarkson M J, Carter S D, Rowan T G, Hammet N C

机构信息

Department of Veterinary Pathology, University of Liverpool, Great Britain.

出版信息

Vet Immunol Immunopathol. 1990 Apr;24(4):361-71. doi: 10.1016/0165-2427(90)90006-e.

DOI:10.1016/0165-2427(90)90006-e

PMID:2339503

Abstract

Analysis of a detergent-solubilized somatic antigen of Ostertagia ostertagi 3rd stage larvae by SDS-PAGE and Western blotting has revealed two specific antigens with apparent molecular weights of 17 and 43 kD under reducing conditions. Probing of the Ostertagia ostertagi preparation with preinfection control sera has shown two cross-reacting antigens with apparent molecular weights of 67 and 81 kD. Both the 17 and the 43 kD antigens were recognised by IgG1 which was the predominant reactive subclass. FPLC fractionation of the crude extract with gel filtration and ion-exchange columns demonstrated immune reactivity in discrete peaks. Comparisons of ELISA and lymphocyte transformation showed antigenic components reactive with both antibodies and primed lymphocytes.

摘要

通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质印迹法对奥斯特他线虫第三期幼虫的去污剂增溶体抗原进行分析，发现在还原条件下有两种表观分子量分别为17kD和43kD的特异性抗原。用感染前对照血清检测奥斯特他线虫制剂，显示出两种表观分子量分别为67kD和81kD的交叉反应抗原。17kD和43kD抗原均被主要的反应性子类IgG1识别。用凝胶过滤和离子交换柱对粗提物进行快速蛋白液相色谱（FPLC）分级分离，在离散峰中显示出免疫反应性。酶联免疫吸附测定（ELISA）和淋巴细胞转化的比较表明，存在与抗体和致敏淋巴细胞均发生反应的抗原成分。