Wedrychowicz H, Holmes P H, Bairden K, Tait A
Department of Parasitology, Warsaw Agricultural University, Poland.
Vet Parasitol. 1994 May;53(1-2):117-32. doi: 10.1016/0304-4017(94)90024-8.
With the use of an enzyme-linked immunosorbent assay (ELISA), it was revealed that surface antigens of both adult and fourth-stage larvae (L4) of Ostertagia circumcincta induced high levels of serum IgG antibodies, while serum IgA antibody levels were low but increased significantly (P < 0.01) after infection. Immunofluorescence studies on the surface of viable L4 and adult nematodes showed that the IgG response was stage-specific only in animals vaccinated with adult surface extracts. The results of Western blot analysis using these antibodies suggested that at least eight polypeptides were shed from the L4 surface to the environment and that infection induced (or boosted) IgG antibody against a further four polypeptides. A comparison of reactivity of pre- and post-infection sera of sheep vaccinated with adult nematode surface antigens suggested that only one of the antigens stripped from the nematode surface was immunogenic and/or present in a concentration sufficient to induce an IgG response following parenteral vaccination. Infection boosted the IgG antibodies to a further four polypeptides. Only one polypeptide of 63 kDa seems to be shed in vivo from the adult nematode surface. Ten to eleven antigens were recognised in adult excretory/secretory products by serum IgG of multiple-infected sheep.
使用酶联免疫吸附测定（ELISA）发现，环形奥斯特他线虫成虫和第四期幼虫（L4）的表面抗原均可诱导高水平的血清IgG抗体，而血清IgA抗体水平较低，但感染后显著升高（P < 0.01）。对活的L4和成虫线虫表面进行的免疫荧光研究表明，仅在用成虫表面提取物免疫的动物中，IgG反应具有阶段特异性。使用这些抗体进行的蛋白质印迹分析结果表明，至少有8种多肽从L4表面释放到环境中，感染诱导（或增强）了针对另外4种多肽的IgG抗体。对用成虫线虫表面抗原免疫的绵羊感染前后血清反应性的比较表明，从线虫表面去除的抗原中只有一种具有免疫原性和/或其浓度足以在经肠胃外免疫后诱导IgG反应。感染使针对另外4种多肽的IgG抗体增强。似乎只有一种63 kDa的多肽在体内从成虫线虫表面脱落。多次感染绵羊的血清IgG在成虫排泄/分泌产物中识别出10至11种抗原。
