Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, Massachusetts, USA.
Nat Methods. 2013 Mar;10(3):243-5. doi: 10.1038/nmeth.2366. Epub 2013 Feb 10.
Artificial activators designed using transcription activator-like effector (TALE) technology have broad utility, but previous studies suggest that these monomeric proteins often exhibit low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy, and guide design of monomeric TALE-based fusion proteins.
人工激活子利用转录激活因子样效应物（TALE）技术设计，具有广泛的应用，但以前的研究表明，这些单体蛋白的活性通常较低。在这里，我们证明 TALE 激活子可以单独或协同组合，在广泛的动态范围内，有效地增强内源性人类基因的表达。这些发现将鼓励 TALE 激活子在研究和治疗中的应用，并指导单体 TALE 基融合蛋白的设计。
