利用降解标签控制集胞藻 PCC 6803 中的蛋白质水平。
Use of degradation tags to control protein levels in the Cyanobacterium Synechocystis sp. Strain PCC 6803.
机构信息
Department of Biology, Washington University, St. Louis, Missouri, USA.
出版信息
Appl Environ Microbiol. 2013 Apr;79(8):2833-5. doi: 10.1128/AEM.03741-12. Epub 2013 Feb 8.
Abstract
We generated a collection of ssrA-based C-terminal protein degradation tags with different degradation strengths. The steady-state fluorescence levels of different enhanced yellow fluorescent protein (eYFP) tag variants in a Synechocystis sp. indicated a tunable range from 1% to 50% of untagged eYFP.
摘要
我们生成了一系列基于 ssrA 的 C 端蛋白降解标签，它们具有不同的降解强度。不同增强型黄色荧光蛋白（eYFP）标签变体在集胞藻 sp.中的稳态荧光水平表明，可调节范围为未标记的 eYFP 的 1%至 50%。
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本文引用的文献
1
SIMPLE CONDITIONS FOR GROWTH OF UNICELLULAR BLUE-GREEN ALGAE ON PLATES(1, 2).平板上单细胞蓝藻生长的简单条件(1, 2)。
J Phycol. 1968 Mar;4(1):1-4. doi: 10.1111/j.1529-8817.1968.tb04667.x.
2
IMG: the Integrated Microbial Genomes database and comparative analysis system.IMG：综合微生物基因组数据库和比较分析系统。
Nucleic Acids Res. 2012 Jan;40(Database issue):D115-22. doi: 10.1093/nar/gkr1044.
3
Design and characterization of molecular tools for a Synthetic Biology approach towards developing cyanobacterial biotechnology.用于开发蓝细菌生物技术的合成生物学方法的分子工具的设计和表征。
Nucleic Acids Res. 2010 May;38(8):2577-93. doi: 10.1093/nar/gkq164. Epub 2010 Mar 17.
4
A one pot, one step, precision cloning method with high throughput capability.一种具有高通量能力的一锅一步精准克隆方法。
PLoS One. 2008;3(11):e3647. doi: 10.1371/journal.pone.0003647. Epub 2008 Nov 5.
5
Global transcriptomic analysis of Cyanothece 51142 reveals robust diurnal oscillation of central metabolic processes.聚球藻51142的全转录组分析揭示了中心代谢过程强烈的昼夜振荡。
Proc Natl Acad Sci U S A. 2008 Apr 22;105(16):6156-61. doi: 10.1073/pnas.0711068105. Epub 2008 Apr 21.
6
Clustal W and Clustal X version 2.0.Clustal W和Clustal X 2.0版本
Bioinformatics. 2007 Nov 1;23(21):2947-8. doi: 10.1093/bioinformatics/btm404. Epub 2007 Sep 10.
7
Engineering controllable protein degradation.工程化可控蛋白质降解
Mol Cell. 2006 Jun 9;22(5):701-7. doi: 10.1016/j.molcel.2006.04.027.
8
A synthetic gene-metabolic oscillator.一种合成基因代谢振荡器。
Nature. 2005 May 5;435(7038):118-22. doi: 10.1038/nature03508.
9
ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences.ARAGORN，一个用于在核苷酸序列中检测tRNA基因和tmRNA基因的程序。
Nucleic Acids Res. 2004 Jan 2;32(1):11-6. doi: 10.1093/nar/gkh152. Print 2004.
10
Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis.ssrA降解标签内重叠的识别决定簇可调节蛋白水解作用。
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10584-9. doi: 10.1073/pnas.191375298. Epub 2001 Sep 4.
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