Mayo Graduate School, Mayo Clinic, Rochester, MN, USA.
Clin Transl Sci. 2013 Feb;6(1):21-5. doi: 10.1111/cts.12025. Epub 2013 Jan 14.
Sepsis biomarker research requires an infrastructure to identify septic patients efficiently and to collect and store specimens properly. We developed a novel infrastructure to study biomarkers of sepsis in children.
Patients in pediatric and neonatal intensive care units were enrolled prospectively; enrollment information was stored in a secure, remotely accessible database. Researchers were notified of electronic medical record (EMR) orders for blood cultures (a surrogate for a diagnostic evaluation of suspected sepsis) by a page triggered by the order. Staff confirmed patient enrollment and remotely submitted an EMR order for collection of study specimens simultaneous with the blood culture. Specimens were processed and stored by a mobile clinical research unit.
Over 2 years, 2029 patients were admitted; 138 were enrolled. Staff received pages for 95% of blood cultures collected from enrolled patients. The median time between the blood culture order and collection was 34 minutes (range 9-241). Study specimens were collected simultaneously with 41 blood cultures. The median times between specimen collection and storage for flow cytometry and cytokine analysis were 33 minutes (range 0-82) and 52 minutes (range 28-98), respectively.
This novel infrastructure facilitated prompt, proper collection and storage of specimens for sepsis biomarker analysis.
脓毒症生物标志物研究需要一种基础设施，以便有效地识别脓毒症患者，并正确地采集和储存标本。我们开发了一种新的基础设施来研究儿童脓毒症的生物标志物。
前瞻性地招募儿科和新生儿重症监护病房的患者；将入组信息存储在一个安全的、远程访问的数据库中。电子病历（EMR）中血培养（疑似脓毒症诊断评估的替代指标）医嘱会触发一个页面，通知研究人员。工作人员确认患者入组，并在与血培养同时远程提交 EMR 医嘱，以采集研究标本。标本由移动临床研究单元进行处理和储存。
在 2 年期间，共收治了 2029 名患者；有 138 名患者入组。工作人员收到了采集入组患者血培养标本的 95%的页面通知。从下达血培养医嘱到采集标本的中位时间为 34 分钟（范围 9-241）。同时采集了 41 份血培养标本进行流式细胞术和细胞因子分析。采集和储存用于流式细胞术和细胞因子分析的标本的中位时间分别为 33 分钟（范围 0-82）和 52 分钟（范围 28-98）。
这种新的基础设施促进了脓毒症生物标志物分析中标本的快速、正确采集和储存。
