Department of Thoracic Surgery, the Third Affiliated-Tumor Hospital, Harbin Medical University, Harbin 150040, China.
Int J Biol Macromol. 2012 Nov;51(4):663-7. doi: 10.1016/j.ijbiomac.2012.06.040. Epub 2012 Jul 3.
Armillaria mellea is a famous traditional Chinese medicinal and edible fungus. In this study, we purified a water-soluble polysaccharide (AMP) from the fruiting bodies of this fungus. AMP contained 94.8% carbohydrate, 2.3% uronic acid and 0.5% protein. Its molecular weight was determined as 4.6 × 10⁵ Da, as determined by high-performance gel-permeation chromatography (HPGPC). Gas chromatography (GC) analysis indicated that AMP was mainly composed of d-glucose. In vitro assay, AMP exhibited a potent tumor growth inhibitory effect on A549 cells, and induced cell cycle disruption in the G0/G1 phase, accompanied by an increment of apoptotic cells. Furthermore, AMP induced the disruption of mitochondrial membrane potential, thus leading to cytochrome c release from mitochondria and activation of caspase-3 and -9. Taken together, our results demonstrate that AMP possesses strong antitumor activities through the mitochondria dependent pathway and activation of caspase cascade through cytochrome c release.
蜜环菌是一种著名的药食两用真菌。在本研究中，我们从该真菌的子实体中纯化得到一种水溶性多糖（AMP）。AMP 含有 94.8%的碳水化合物、2.3%的糖醛酸和 0.5%的蛋白质。采用高效凝胶渗透色谱（HPGPC）测定其分子量为 4.6×105 Da。气相色谱（GC）分析表明，AMP 主要由 d-葡萄糖组成。体外实验表明，AMP 对 A549 细胞具有显著的肿瘤生长抑制作用，并诱导细胞周期停滞在 G0/G1 期，同时伴有凋亡细胞的增加。此外，AMP 诱导线粒体膜电位破坏，导致细胞色素 c 从线粒体释放，并激活 caspase-3 和 caspase-9。综上所述，我们的研究结果表明，AMP 通过线粒体依赖性途径和细胞色素 c 释放激活 caspase 级联反应发挥强大的抗肿瘤活性。
