Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682 041, India.
Carbohydr Polym. 2013 Feb 15;92(2):1561-5. doi: 10.1016/j.carbpol.2012.10.041. Epub 2012 Oct 23.
In this work, Hemigraphis alternata extract incorporated chitosan scaffold was synthesized and characterized for wound healing. The antibacterial activity of Hemigraphis incorporated chitosan scaffold (HIC) against Escherichia coli and Staphylococcus aureus was evaluated which showed a reduction in total colony forming units by 45-folds toward E. coli and 25-fold against S. aureus respectively. Cell viability studies using Human Dermal Fibroblast cells (HDF) showed 90% viability even at 48 h when compared to the chitosan control. The herbal scaffold made from chitosan was highly haemostatic and antibacterial. The obtained results were in support that the herbal scaffold can be effectively applied for infectious wounds.
在这项工作中，合成并表征了含有扁担杆提取物的壳聚糖支架，用于伤口愈合。评估了含有扁担杆的壳聚糖支架（HIC）对大肠杆菌和金黄色葡萄球菌的抗菌活性，结果表明，HIC 对大肠杆菌的总菌落形成单位减少了 45 倍，对金黄色葡萄球菌减少了 25 倍。用人类真皮成纤维细胞（HDF）进行的细胞活力研究表明，与壳聚糖对照相比，即使在 48 小时时，细胞活力仍高达 90%。由壳聚糖制成的草药支架具有高度的止血和抗菌作用。研究结果表明，草药支架可有效应用于感染性伤口。
