Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi-682 041, India.
Carbohydr Polym. 2013 Feb 15;92(2):1470-6. doi: 10.1016/j.carbpol.2012.10.058. Epub 2012 Nov 9.
In this work chitosan-hyaluronan composite sponge incorporated with chondroitin sulfate nanoparticle (nCS) was developed. The fabrication of hydrogel was based on simple ionic cross-linking using EDC, followed by lyophilization to obtain the composite sponge. nCS suspension was characterized using DLS and SEM and showed a size range of 100-150 nm. The composite sponges were characterized using SEM, FT-IR and TG-DTA. Porosity, swelling, biodegradation, blood clotting and platelet activation of the prepared sponges were also evaluated. Nanocomposites showed a porosity of 67% and showed enhanced swelling and blood clotting ability. Cytocompatibility and cell adhesion studies of the sponges were done using human dermal fibroblast (HDF) cells and the nanocomposite sponges showed more than 90% viability. Nanocomposite sponges also showed enhanced proliferation of HDF cells within two days of study. These results indicated that this nanocomposite sponges would be a potential candidate for wound dressing.
在这项工作中，制备了一种壳聚糖-透明质酸复合海绵，其中掺入了硫酸软骨素纳米粒子（nCS）。水凝胶的制备基于使用 EDC 的简单离子交联，然后进行冷冻干燥以获得复合海绵。使用 DLS 和 SEM 对 nCS 悬浮液进行了表征，其粒径范围为 100-150nm。使用 SEM、FT-IR 和 TG-DTA 对复合海绵进行了表征。还评估了制备的海绵的孔隙率、溶胀、生物降解、凝血和血小板激活。纳米复合材料的孔隙率为 67%，表现出增强的溶胀和凝血能力。使用人真皮成纤维细胞（HDF）细胞进行了海绵的细胞相容性和细胞黏附研究，纳米复合材料海绵的活力超过 90%。在两天的研究中，纳米复合材料海绵还显示出 HDF 细胞的增殖增强。这些结果表明，这种纳米复合材料海绵将是一种有潜力的伤口敷料候选物。
