Department of Chemistry, Avinashilingam Institute for Home Science and Higher Education for Women, 641043, Coimbatore, India.
Appl Biochem Biotechnol. 2021 Oct;193(10):3232-3252. doi: 10.1007/s12010-021-03600-1. Epub 2021 Jun 10.
Skin substitutes are a restorative material used to treat many skin injuries by replacing or masking the wound. It is also capable of producing an original skin type. In this study, gold nanoparticle-aided skin substitutes were prepared using biodegradable materials (chitosan, sodium alginate, and gelatin) under the magnetic stirring method. Gold ions were reduced using aqueous extract of Cyperus rotundus and Hemigraphis alternata. The formation of prepared gold nanoparticles was confirmed using spectroscopy techniques. The physical parameters of the skin substitutes were tested, and it was characterized using FTIR, DTG, laser profilometer, and FESEM analysis. HAaNP-aided skin substitutes have a bubble-like texture, and it facilitates higher water-absorbing ability. CRaNP aided skin substitutes reducing the hydrophilicity of the prepared skin substitutes. Antioxidant and antifungal skin substitute activities were carried out using DPPH radical scavenging activity and disk diffusion method, respectively. The antioxidant activity revealed the skin substitutes to possess significant free radical inhibition and as the number of gold nanoparticles increases, the activity also increases. The prepared samples show excellent activity against Aspergillus niger. The MTT assay reveals that the cancer cell (A-375) viability decreases by increasing skin substitutes' concentration. The normal cells (HEK-293) were cultured in a medium containing skin substitutes, facilitating the growth of cells. The cell attachment was observed in prepared cell lines after 24-h treatment. The results of this study suggest the prepared Cyperus rotundus and Hemigraphis alternata embedded with gold nanoparticle-aided skin substitutes are a promising material for medical and cosmetic application.
皮肤替代物是一种修复材料，通过替代或掩盖伤口来治疗许多皮肤损伤。它还能够产生原始的皮肤类型。在这项研究中，使用可生物降解材料（壳聚糖、海藻酸钠和明胶）在磁力搅拌法下制备了金纳米粒子辅助的皮肤替代物。使用香附和平贝母的水提物还原金离子。使用光谱技术确认了制备的金纳米粒子的形成。测试了皮肤替代物的物理参数，并使用 FTIR、DTG、激光轮廓仪和 FESEM 分析对其进行了表征。HAaNP 辅助的皮肤替代物具有气泡状的质地，这使其具有更高的吸水性。CRaNP 辅助的皮肤替代物降低了制备的皮肤替代物的亲水性。通过 DPPH 自由基清除活性和圆盘扩散法分别进行抗氧化和抗真菌皮肤替代物活性测试。抗氧化活性表明，皮肤替代物具有显著的自由基抑制作用，并且随着金纳米粒子数量的增加，活性也增加。制备的样品对黑曲霉表现出优异的活性。MTT 测定法显示，随着皮肤替代物浓度的增加，癌细胞（A-375）的活力降低。将皮肤替代物添加到培养基中培养正常细胞（HEK-293），促进细胞生长。在 24 小时处理后，在制备的细胞系中观察到细胞附着。这项研究的结果表明，香附和平贝母嵌入金纳米粒子辅助的皮肤替代物是一种有前途的医学和化妆品应用材料。
