Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Carbohydr Polym. 2022 Feb 15;278:118926. doi: 10.1016/j.carbpol.2021.118926. Epub 2021 Nov 26.
Skin tissue engineering is an advanced method to repair and regenerate skin injuries. Recent research is focused on the development of scaffolds that are safe, bioactive, and cytocompatible. In this work, a new hybrid nanofibrous scaffold composed of polycaprolactone/chitosan-polyethylene oxide (PCL/Cs-PEO) incorporated with Arnebia euchroma (A. euchroma) extract were synthesized by the two-nozzle electrospinning method. Then the synthesized scaffold was characterized for morphology, sustainability, chemical structure and properties. Moreover, to verify their potential in the burn wound healing process, biodegradation rate, contact angle, swelling properties, water vapor permeability, mechanical properties, antibacterial activity and drug release profile were measured. Furthermore, cytotoxicity and biocompatibility tests were performed on human dermal fibroblasts cell line via XTT and LDH assay. It is shown that the scaffold improved and increased proliferation during in-vitro studies. Thus, results confirm the efficacy and potential of the hybrid nanofibrous scaffold for skin tissue engineering.
皮肤组织工程是一种修复和再生皮肤损伤的先进方法。最近的研究集中在开发安全、生物活性和细胞相容性的支架上。在这项工作中，通过双喷头静电纺丝法合成了一种由聚己内酯/壳聚糖-聚氧化乙烯（PCL/Cs-PEO）与红花（A. euchroma）提取物复合的新型杂化纳米纤维支架。然后对合成的支架进行形貌、可持续性、化学结构和性能的表征。此外，为了验证其在烧伤创面愈合过程中的潜力，测量了降解速率、接触角、溶胀性能、水蒸气透过率、机械性能、抗菌活性和药物释放曲线。此外，通过 XTT 和 LDH 测定法对人皮肤成纤维细胞系进行了细胞毒性和生物相容性测试。结果表明，支架在体外研究中提高和促进了细胞增殖。因此，结果证实了杂化纳米纤维支架在皮肤组织工程中的功效和潜力。
