Dept° de Física, Química e Biologia, Universidade Estadual Paulista - UNESP, CEP 19060-900, Presidente Prudente, São Paulo, Brazil.
Carbohydr Polym. 2013 Feb 15;92(2):1908-14. doi: 10.1016/j.carbpol.2012.10.034. Epub 2012 Nov 14.
An exocellular β-(1→6)-D-glucan (lasiodiplodan) produced by a strain of Lasiodiplodia theobromae (MMLR) grown on sucrose was derivatized by sulfonation to promote anticoagulant activity. The structural features of the sulfonated β-(1→6)-D-glucan were investigated by UV-vis, FT-IR and (13)C NMR spectroscopy, and the anticoagulant activity was investigated by the classical coagulation assays APTT, PT and TT using heparin as standard. The content of sulfur and degree of substitution of the sulfonated glucan was 11.73% and 0.95, respectively. UV spectroscopy showed a band at 261 nm due to the unsaturated bond formed in the sulfonation reaction. Results of FT-IR and (13)C NMR indicated that sulfonyl groups were inserted on the polysaccharide. The sulfonated β-(1→6)-D-glucan presented anticoagulant activity as demonstrated by the increase in dose dependence of APTT and TT, and these actions most likely occurred because of the inserted sulfonate groups on the polysaccharide. The lasiodiplodan did not inhibit the coagulation tests.
一种由胶孢炭疽菌（MMLR）在蔗糖上生长产生的胞外 β-（1→6）-D-葡聚糖（拉索多糖）经过磺化衍生化以提高抗凝活性。通过紫外可见光谱、傅里叶变换红外光谱和（13）C NMR 光谱研究了磺化 β-（1→6）-D-葡聚糖的结构特征，并以肝素为标准，通过经典的凝血测定 APTT、PT 和 TT 研究了抗凝活性。磺化葡聚糖的硫含量和取代度分别为 11.73%和 0.95。紫外光谱显示由于磺化反应中形成的不饱和键在 261nm 处出现一个带。傅里叶变换红外光谱和（13）C NMR 的结果表明，磺酰基被插入到多糖中。磺化 β-（1→6）-D-葡聚糖表现出抗凝活性，这表现为 APTT 和 TT 的剂量依赖性增加，这些作用很可能是因为多糖上插入的磺酸盐基团。拉索多糖没有抑制凝血试验。
