Departamento de Ciências Biológicas, Universidade Estadual Paulista - UNESP (FCL-Assis), CEP 19806-900 Assis, SP, Brazil.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980 Curitiba, PR, Brazil.
Carbohydr Polym. 2015 Nov 20;133:539-46. doi: 10.1016/j.carbpol.2015.07.060. Epub 2015 Jul 26.
The chemical composition and structural characterization of exopolysaccharides from the fungus Lasiodiplodia theobromae MMBJ are described, and the immunomodulatory activity of a purified β-glucan was evaluated. L. theobromae MMBJ produced three different β-glucans. One, fraction PEPS, was a branched (1→3)(1→6)-β-glucan and was insoluble in cold water. The other two, fractions SEPS-005R and SEPS-10E, were characterized as linear (1→6)-β-glucans with molar mass of 1.8×10(6)Da and 7.0×10(3)Da, respectively. From a total of 2.2g/L of EPS produced by L. theobromae through submerged fermentation, 1.5g/L (67%) was of the branched (1→3)(1→6)-β-glucan, while 25% (w/w) were linear (1→6)-β-glucans. Tests conducted with macrophages showed that the high molar mass (1→6)-β-glucan fraction (SEPS-005R) induced a pro-inflammatory response pattern.
文中描述了从真菌胶孢炭疽菌 MMBJ 中提取的胞外多糖的化学成分和结构特征，并评价了一种纯化的β-葡聚糖的免疫调节活性。胶孢炭疽菌 MMBJ 产生了三种不同的β-葡聚糖。一种是分支（1→3）（1→6）-β-葡聚糖，称为 PEPS 部分，不溶于冷水。另外两种，SEPS-005R 和 SEPS-10E 部分，被鉴定为具有 1.8×10(6)Da 和 7.0×10(3)Da 摩尔质量的线性（1→6）-β-葡聚糖。从胶孢炭疽菌通过浸没发酵生产的总共 2.2g/L 的 EPS 中，有 1.5g/L（67%）为分支（1→3）（1→6）-β-葡聚糖，而 25%（w/w）为线性（1→6）-β-葡聚糖。用巨噬细胞进行的测试表明，高摩尔质量（1→6）-β-葡聚糖部分（SEPS-005R）诱导了促炎反应模式。
