Discipline of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, G.B. Marg, Bhavnagar, Gujarat 364002, India.
Carbohydr Polym. 2013 Feb 15;92(2):1942-5. doi: 10.1016/j.carbpol.2012.11.055. Epub 2012 Nov 29.
Salicornia brachiata is an extreme halophyte, growing opulently in salt marshes and considered as potential alternative crop for seawater agriculture. Salicornia seeds are rich in protein and tender-shoots are eaten as salad greens. Total cell wall carbohydrate was isolated from seedlings, digested with β-glucanase enzyme cocktail and oligosaccharide mass profiling (OLIMP) was performed by using MALDI TOF-TOF mass spectroscopy. Salicornia OLIMP is represented by characteristic mass peaks m/z 477.3297-2094.4363. MS spectra exhibit xyloglucan oligosaccharide building blocks, dominated by XXXG (1084.9927 m/z). Characteristic mass peaks (m/z) of xyloglucan oligomers (XXG, XXFG, XLXG and XLFG) were also detected, which showed resemblance to the mass profile of highly nutritious plant soybean. The present study is the first report on OLIMP for any edible halophyte, so far. OLIMP supports use of Salicornia as a potential source of dietary supplementation. Further linkage-analysis is required to get the structural information of oligosaccharides.
滨藜是一种极端盐生植物，在盐沼中繁茂生长，被认为是海水农业的潜在替代作物。滨藜种子富含蛋白质，嫩枝可作为沙拉食用。从幼苗中分离出总细胞壁碳水化合物，用β-葡聚糖酶酶混合物进行消化，并通过 MALDI TOF-TOF 质谱进行寡糖质量分析（OLIMP）。滨藜的 OLIMP 由特征质量峰 m/z 477.3297-2094.4363 表示。MS 光谱显示出木葡聚糖寡糖构建块，主要由 XXXG（1084.9927 m/z）组成。还检测到木葡聚糖低聚糖（XXG、XXFG、XLXG 和 XLFG）的特征质量峰（m/z），这与高营养植物大豆的质量谱相似。目前的研究是迄今为止对任何可食用盐生植物进行 OLIMP 的首次报道。OLIMP 支持将滨藜用作膳食补充剂的潜在来源。需要进一步的连锁分析来获取寡糖的结构信息。
