Division of Marine Biotechnology and Ecology, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar (Gujarat), India.
Sci Rep. 2016 Aug 23;6:31686. doi: 10.1038/srep31686.
A salt- and drought-responsive novel gene SbSDR1 is predominantly localised to the nucleus, up-regulated under abiotic stresses and is involved in the regulation of metabolic processes. SbSDR1 showed DNA-binding activity to genomic DNA, microarray analysis revealed the upregulation of host stress-responsive genes and the results suggest that SbSDR1 acts as a transcription factor. Overexpression of SbSDR1 did not affect the growth and yield of transgenic plants in non-stress conditions. Moreover, the overexpression of SbSDR1 stimulates the growth of plants and enhances their physiological status by modulating the physiology and inhibiting the accumulation of reactive oxygen species under salt and osmotic stress. Transgenic plants that overexpressed SbSDR1 had a higher relative water content, membrane integrity and concentration of proline and total soluble sugars, whereas they showed less electrolyte leakage and lipid peroxidation than wild type plants under stress conditions. In field conditions, SbSDR1 plants recovered from stress-induced injuries and could complete their life cycle. This study suggests that SbSDR1 functions as a molecular switch and contributes to salt and osmotic tolerance at different growth stages. Overall, SbSDR1 is a potential candidate to be used for engineering salt and drought tolerance in crops without adverse effects on growth and yield.
一个对盐和干旱响应的新型基因 SbSDR1 主要定位于细胞核，在非生物胁迫下表达上调，参与代谢过程的调节。SbSDR1 显示与基因组 DNA 的 DNA 结合活性，微阵列分析显示宿主应激响应基因的上调，结果表明 SbSDR1 作为转录因子发挥作用。SbSDR1 的过表达在非胁迫条件下不会影响转基因植物的生长和产量。此外，SbSDR1 的过表达通过调节植物的生理机能和抑制活性氧的积累，刺激植物的生长并增强其生理状态，从而提高植物在盐和渗透胁迫下的生长。过表达 SbSDR1 的转基因植物具有更高的相对含水量、膜完整性和脯氨酸及总可溶性糖的浓度，而在胁迫条件下，它们表现出比野生型植物更少的电解质泄漏和脂质过氧化。在田间条件下，SbSDR1 植物从胁迫诱导的损伤中恢复过来，并能完成其生命周期。本研究表明，SbSDR1 作为一个分子开关，在不同的生长阶段对盐和渗透胁迫具有耐受性。总的来说，SbSDR1 是一个有潜力的候选基因，可以用于工程作物的耐盐耐旱性，而不会对生长和产量产生不利影响。
