College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Taian 271018, PR China.
Chemosphere. 2013 May;91(8):1107-12. doi: 10.1016/j.chemosphere.2013.01.013. Epub 2013 Feb 8.
Ionic liquids were recently found to be toxic to aquatic organisms. Therefore, the present study investigated the effects of 1-decyl-3-methylimidazolium bromide ([C10mim]Br) on oxidative stress and DNA damage in zebrafish. Male and female zebrafish were separated and exposed to five concentrations of [C10mim]Br (0, 5, 10, 20, and 40 mg L(-1)) and were sampled on days 7, 14, 21 and 28. Compared to control groups, the activities of antioxidant enzymes were significantly decreased at most exposure intervals. This decreased activity resulted in the production of excess reactive oxygen species (ROS) and increased malondialdehyde (MDA) content in zebrafish liver. Additionally, it was noteworthy that a clear dose-response was found for DNA damage. As for sex differences, significant differences in catalase (CAT) and ROS were found on the 7th day. In conclusion, the exposure of [C10mim]Br caused DNA damage, leading to antioxidant responses in zebrafish livers.
离子液体最近被发现对水生生物具有毒性。因此，本研究调查了 1-癸基-3-甲基咪唑溴 ([C10mim]Br) 对斑马鱼氧化应激和 DNA 损伤的影响。雄性和雌性斑马鱼分开暴露于五种浓度的 [C10mim]Br（0、5、10、20 和 40mg/L），并在第 7、14、21 和 28 天取样。与对照组相比，大多数暴露时间点的抗氧化酶活性显著降低。这种活性降低导致过量的活性氧 (ROS) 的产生和斑马鱼肝中丙二醛 (MDA) 含量的增加。此外，值得注意的是，发现 DNA 损伤存在明显的剂量反应关系。至于性别差异，第 7 天发现 CAT 和 ROS 有显著差异。总之，[C10mim]Br 的暴露导致了 DNA 损伤，从而导致斑马鱼肝脏中的抗氧化反应。
