Zhang Cheng, Shao Yuting, Zhu Lusheng, Wang Jinhua, Wang Jun, Guo Yingying
College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agricultural University, Taian 271018, China.
Environ Toxicol Pharmacol. 2017 Apr;51:131-137. doi: 10.1016/j.etap.2017.02.018. Epub 2017 Feb 21.
The present study examined the potential toxicity of 1-butyl-3-methylimidazolium chloride ([Bmim]Cl) and 1-butyl-3- methylimidazolium tetrafluoroborate ([Bmim]BF) in the liver cells of zebrafish (Danio rerio) with different doses (20-160mg/L) on 7 and 14days. The effects of [Bmim]Cl and [Bmim]BF on acute toxicity, reactive oxygen species (ROS), antioxidant enzymes, glutathione S-transferase (GST), malondialdehyde (MDA), and DNA damage degree in livers of zebrafish were determined. The 50% lethal concentration (LC) values after a 96-h exposure to [Bmim]Cl and [Bmim]BF were 632.8±67.4 and 604.6±56.2mg/L, respectively, which indicated that the substances were practically harmless. The minor discrepancy may be caused by the different anions. The ROS levels were dose-dependent, which may cause the inhibition of antioxidant enzyme activity, lipid peroxidation, DNA damage and the stimulation of detoxifying enzyme activity. The present study can also provide scientific support for the future selection and evaluation of ionic liquids (ILs).
本研究检测了不同剂量（20 - 160mg/L）的1-丁基-3-甲基咪唑氯盐（[Bmim]Cl）和1-丁基-3-甲基咪唑四氟硼酸盐（[Bmim]BF）在7天和14天时对斑马鱼（Danio rerio）肝细胞的潜在毒性。测定了[Bmim]Cl和[Bmim]BF对斑马鱼肝脏急性毒性、活性氧（ROS）、抗氧化酶、谷胱甘肽S-转移酶（GST）、丙二醛（MDA）以及DNA损伤程度的影响。暴露于[Bmim]Cl和[Bmim]BF 96小时后的50%致死浓度（LC）值分别为632.8±67.4和604.6±56.2mg/L，这表明这些物质实际无害。微小差异可能由不同阴离子引起。ROS水平呈剂量依赖性，这可能导致抗氧化酶活性受到抑制、脂质过氧化、DNA损伤以及解毒酶活性受到刺激。本研究也可为未来离子液体（ILs）的选择和评估提供科学支持。
