National Engineering Laboratory for Efficient Use of Soil and Fertilizer Resources, Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, 61 Daizong Road, Taian, 271018, People's Republic of China.
Arch Environ Contam Toxicol. 2014 Aug;67(2):261-9. doi: 10.1007/s00244-014-0046-2. Epub 2014 Jun 8.
Ionic liquids (ILs) have a great reputation due to their negligible volatility, designability, good stability, and ability to be recycled. They are considered to be "green" solvents and have great promise in many fields. In recent years, the toxicities of ILs have garnered increasing attention as reported by a number of studies. However, previous studies have primarily focused on their lethal toxicities, and data were limited on their toxic effects at nonlethal doses. We performed a study on the toxic effects of 1-octyl-3-methylimidazolium bromide ([Omim]Br) on zebrafish. During a 28-day period, male and female zebrafish were separately exposed to sequential concentrations (0, 5, 10, 20, and 40 mg/L) of [Omim]Br. Fishes were sampled after 7, 14, 21, and 28 days of exposure, and reactive oxygen species (ROS) levels, activities of antioxidant enzymes (superoxide dismutase and catalase), lipid peroxidation (LPO), and DNA damage in fish livers were measured. ROS, LPO, and DNA damage were all induced by the ionic liquid, and antioxidant enzyme activities increased at the beginning and then decreased. These phenomena demonstrate that [Omim]Br can induce oxidative stress and DNA damage in zebrafish.
离子液体（ILs）由于其挥发性低、可设计性、良好的稳定性和可回收性而备受关注。它们被认为是“绿色”溶剂，在许多领域具有广阔的应用前景。近年来，越来越多的研究报告称离子液体具有毒性。然而，以前的研究主要集中在它们的致死毒性上，关于非致死剂量下的毒性效应的数据有限。我们对 1-辛基-3-甲基咪唑溴盐（[Omim]Br）对斑马鱼的毒性效应进行了研究。在 28 天的时间里，雄性和雌性斑马鱼分别暴露于[Omim]Br 的连续浓度（0、5、10、20 和 40mg/L）中。暴露 7、14、21 和 28 天后取样，测量鱼类肝脏中的活性氧（ROS）水平、抗氧化酶（超氧化物歧化酶和过氧化氢酶）活性、脂质过氧化（LPO）和 DNA 损伤。离子液体诱导了 ROS、LPO 和 DNA 损伤，抗氧化酶活性先升高后降低。这些现象表明[Omim]Br 可诱导斑马鱼产生氧化应激和 DNA 损伤。
