在克氏锥虫病传播媒介三带喙库蚊（半翅目：红蝽科）中线粒体 NADH 脱氢酶亚单位 5 和 NADH 脱氢酶亚单位 4 基因的变异。
Variation in mitochondrial NADH dehydrogenase subunit 5 and NADH dehydrogenase subunit 4 genes in the Chagas disease vector Triatoma infestans (Hemiptera: Reduviidae).
机构信息
Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, INICSA (CONICET-Universidad Nacional de Córdoba), Ciudad Universitaria, Córdoba, Argentina.
出版信息
Am J Trop Med Hyg. 2013 May;88(5):893-6. doi: 10.4269/ajtmh.12-0451. Epub 2013 Feb 11.
Variation in mitochondrial NADH dehydrogenase subunit 5 (ND5) and NADH dehydrogenase subunit 4 (ND4) genes was surveyed in Triatoma infestans from 24 localities of Argentina. The DNA sequence comparisons of 2,183 basepairs of the mitochondrial genome, which include the complete sequence of ND5 (1,712 basepairs) and 401 basepairs of ND4 genes, showed 19 haplotypes determined by 48 variable sites and a nucleotide diversity value of 0.292%. Twenty-six (65%) substitutions were synonymous, and there were 14 (35%) predicted amino acid replacements in ND5. In ND4, 5 (62.5%) substitutions were synonymous and 3 (37.5%) were replacement sites. Samples from six localities studied shared one haplotype and the rest of the localities had different haplotypes. The amplified regions should be useful for population genetic studies.
对来自阿根廷 24 个地点的 24 个特里亚托马 infestans 进行了线粒体 NADH 脱氢酶亚单位 5（ND5）和 NADH 脱氢酶亚单位 4（ND4）基因的变异调查。对包括 ND5（1712 个碱基对）全长序列和 ND4 基因 401 个碱基对的线粒体基因组 2183 个碱基对的 DNA 序列比较显示，有 19 个由 48 个可变位点决定的单倍型和 0.292%的核苷酸多样性值。26 个（65%）替换是同义的，ND5 中有 14 个（35%）预测的氨基酸替换。在 ND4 中，5 个（62.5%）替换是同义的，3 个（37.5%）是替换位点。来自六个研究地点的样本共享一个单倍型，其余地点则有不同的单倍型。扩增区域应该对种群遗传研究有用。
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