Wang Chia-Chen, Huang Chuen-Lin, Lee Shao-Chen, Sue Yuh-Mou, Leu Fur-Jiang
Department of Dermatology, Cardinal Tien Hospital, New Taipei City 231, Taiwan.
Lasers Surg Med. 2013 Feb;45(2):116-22. doi: 10.1002/lsm.22086. Epub 2013 Feb 8.
Cosmetic tattoos are difficult to treat using Q-switched lasers. We introduce a novel method for the treatment of cosmetic tattoos using a nonablative fractional laser and investigate the underlying pathophysiological mechanisms in an animal model.
STUDY DESIGN/MATERIALS AND METHODS: Ten rats were tattooed on their backs with white and flesh-colored pigments. One-half of each tattoo was treated with a 1,550-nm, erbium:glass fractional laser system with energy settings of 17 mJ and 169 MTZ/cm(2) × 2 passes for five sessions at 1-month intervals. The untreated half of each tattoo served as the control. An independent physician reviewed the photographs and scored the clinical response. Serial skin samples were obtained at baseline and at various times after laser treatment. These tissue sections were stained with hematoxylin and eosin, and immunostained for types I, III, and IV collagen; laminin; fibronectin; and α-smooth muscle actin.
White tattoos showed excellent responses in two rats and good responses in eight rats, whereas flesh-colored tattoos showed excellent responses in four rats and good responses in six rats (P = 0.001 in both cases compared with baseline). Both tattoos exhibited a similar clearance rate (P > 0.05) and histological reactions. Microscopic epidermal necrotic debris (MEND) containing tattoo pigments and collagen fibrils appeared on day 1, increased on day 2, and was exfoliated after 5 days. The dermal-epidermal junction lost integrity 30 minutes after treatment, but recovered completely on day 3. The expression of fibronectin and collagen-III, which play key roles in wound healing, increased around the microscopic treatment zone on days 1-5 and 4-7, respectively. A few myofibroblasts appeared on days 4-7.
Nonablative fractional lasers (NAFLs) successfully remove cosmetic tattoos by transepidermal elimination of tattoo pigments through the disrupted dermal-epidermal junction. This action is facilitated by the wound healing process.
调Q激光治疗美容纹身存在困难。我们介绍一种使用非剥脱性分数激光治疗美容纹身的新方法，并在动物模型中研究其潜在的病理生理机制。
研究设计/材料与方法：在10只大鼠背部用白色和肉色颜料进行纹身。每个纹身的一半用波长1550nm的铒玻璃分数激光系统治疗，能量设置为17mJ，光斑密度为169MTZ/cm²，照射2遍，共进行5次治疗，每次间隔1个月。每个纹身未治疗的一半作为对照。由一名独立的医生查看照片并对临床反应进行评分。在基线和激光治疗后的不同时间获取连续的皮肤样本。这些组织切片用苏木精和伊红染色，并进行I型、III型和IV型胶原、层粘连蛋白、纤连蛋白以及α平滑肌肌动蛋白的免疫染色。
白色纹身有2只大鼠反应极佳，8只大鼠反应良好；而肉色纹身有4只大鼠反应极佳，6只大鼠反应良好（与基线相比，两种情况P均 = 0.001）。两种纹身的清除率相似（P > 0.），组织学反应也相似。含纹身颜料和胶原纤维的微小表皮坏死碎屑（MEND）在第1天出现，第2天增多，5天后脱落。治疗后30分钟真皮表皮连接完整性丧失，但在第3天完全恢复。在伤口愈合中起关键作用的纤连蛋白和III型胶原的表达分别在第1 - 5天和第4 - 7天在微小治疗区域周围增加。在第4 - 7天出现少量肌成纤维细胞。
非剥脱性分数激光通过经皮消除穿过破坏的真皮表皮连接的纹身颜料成功去除美容纹身。伤口愈合过程促进了这一作用。
